Recombinant
RabMAb

Recombinant Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (Alexa Fluor® 647) (ab237374)

Overview

  • Product name

    Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (Alexa Fluor® 647)
    See all Histone H3 primary antibodies
  • Description

    Rabbit monoclonal [EPR20358-120] to Histone H3 (citrulline R17) (Alexa Fluor® 647)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Histone H3 aa 1-100 (citrulline R17). The exact sequence is proprietary.
    Database link: P68431

  • Positive control

    • ICC/IF: HEK-293T cells transfected with a GFP-tagged PADI4 expression construct, then treated with 10mM CaCl2 for 2 hours and 10µM Ionomycin for 2 hours.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20358-120
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab237374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

Target

  • Relevance

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post- modifications of histones, also called histone code, and nucleosome remodeling. P68431 and Q71DI3 are expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation. Q16695 is expressed in tesicular cells. Q6NXT2 is specifically expressed in the seminiferous tubules of testis and this Hominid-specific H3.5/H3F3C preferentially colocalizes with euchromatin, and it is associated with actively transcribed genes. P84243 is a variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Post-translational modification. Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • Cit Histone H3 antibody
    • Citrullated antibody
    • citrullin antibody
    • Citrullinated Histone H3 antibody
    • citrulline antibody
    • H3 3 like sequence MH921 antibody
    • H3 3A antibody
    • H3 histone antibody
    • H3 histone family member E pseudogene antibody
    • H3F3 antibody
    • HIST3H3 antibody
    • Histone H3 3 pseudogene antibody
    • Histone H3 Citrullated antibody
    • Histone H3.1 antibody
    • Histone H3.1t antibody
    • Histone H3.2 antibody
    • Histone H3.3 antibody
    • Histone H3.3C antibody
    • Histone H3.5 antibody
    see all

Images

  • Immunofluorescent analysis of 4% PFA-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labeling Histone H3 with ab237374 at 1/100 dilution (red). Confocal image showing nuclear staining in HEK-293T cells transfected with a GFP-tagged PADI4 expression construct (green), then treated with 10mM CaCl2 for 2 hours and 10µM Ionomycin for 2 hours. Nuclear counterstain is DAPI (blue).

References

ab237374 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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