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Epigenetics and Nuclear Signaling Histones H3 Citrullated
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Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)

  • Datasheet
  • SDS
Reviews (24)Q&A (7)References (250)

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Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
  • Immunocytochemistry - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
  • Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
  • Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
  • Peptide Array - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
  • Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)

Key features and details

  • Rabbit polyclonal to Histone H3 (citrulline R2 + R8 + R17)
  • Suitable for: PepArr, ICC, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (ab219406)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Histone H3 (citrulline R2 + R8 + R17) antibody
    See all Histone H3 primary antibodies
  • Description

    Rabbit polyclonal to Histone H3 (citrulline R2 + R8 + R17)
  • Host species

    Rabbit
  • Specificity

    ab5103 detects a 17 kDa band in single lane Western Blot. Peptide inhibition in Western Blot hasn't been processed. Modification specificity is determined by Peptide Array. ab5103 binds strongly to Histone H3 citrulline 2 + 8 + 17 peptide.
  • Tested Applications & Species

    Application Species
    ICC
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Histone H3 aa 1-100 (citrulline R2 + R8 + R17) conjugated to keyhole limpet haemocyanin. Also SwissProt: P84243, Q71DI3, Q16695, Q6NXT2.
    Database link: P68431
    (Peptide available as ab32876)

  • Positive control

    • WB: Mouse and rat brain tissue lysates. HL60 + DMSO, NIH/3T3 and PC12 whole cell lysates. ICC: MCF7 cells.
  • General notes

    Immunizing Peptide (Blocking) - ab32876.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Citrullated

Associated products

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human Histone H3 (citrulline R2 + R8 + R17) peptide (ab32876)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Histone H3 protein (Active) (ab198757)
  • Related Products

    • Anti-Histone H3 antibody [mAbcam 10799] - ChIP Grade (ab10799)
    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220)
    • Human Histone H3 (tri methyl K4) peptide (ab1342)
    • Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (ab14955)
    • Human Histone H3 (tri methyl K9) peptide (ab1773)
    • Human Histone H3 (tri methyl K27) peptide (ab1782)
    • Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002)
    • Human Histone H3 (unmodified) peptide (ab7228)
    • Human Histone H3 (di methyl K4) peptide (ab7768)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab5103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC
Human
WB
Mouse
Rat
Human
All applications
Rabbit
Cow
Monkey
a wide range of other species
Application Abreviews Notes
PepArr
Use a concentration of 0.2 - 0.02 µg/ml.
ICC
Use a concentration of 1 µg/ml.
WB (9)
Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).

In our hands, when tested in western blot, this product typically gives a weaker signal in mouse and rat tissue lysates compared to mouse and rat cell lines. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

Abcam recommends using 3-5% milk as the blocking agent

We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody.

Notes
PepArr
Use a concentration of 0.2 - 0.02 µg/ml.
ICC
Use a concentration of 1 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).

In our hands, when tested in western blot, this product typically gives a weaker signal in mouse and rat tissue lysates compared to mouse and rat cell lines. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

Abcam recommends using 3-5% milk as the blocking agent

We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody.

Target

  • Function

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities

    Belongs to the histone H3 family.
  • Developmental stage

    Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
  • Post-translational
    modifications

    Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
    Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
    Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
    Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
    Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
    Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
  • Cellular localization

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P68431 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8350 Human
    • Entrez Gene: 8351 Human
    • Entrez Gene: 8352 Human
    • Entrez Gene: 8353 Human
    • Entrez Gene: 8354 Human
    • Entrez Gene: 8355 Human
    • Entrez Gene: 8356 Human
    • Entrez Gene: 8357 Human
    • Entrez Gene: 8358 Human
    • Entrez Gene: 8968 Human
    • Entrez Gene: 319152 Mouse
    • Entrez Gene: 319153 Mouse
    • Entrez Gene: 360198 Mouse
    • Entrez Gene: 97908 Mouse
    • Entrez Gene: 100364501 Rat
    • Entrez Gene: 100365669 Rat
    • Entrez Gene: 291159 Rat
    • Entrez Gene: 679994 Rat
    • Entrez Gene: 680511 Rat
    • Entrez Gene: 682330 Rat
    • Omim: 602810 Human
    • SwissProt: P68432 Cow
    • SwissProt: P68431 Human
    • SwissProt: P68433 Mouse
    • SwissProt: Q6LED0 Rat
    • Unigene: 132854 Human
    • Unigene: 247813 Human
    • Unigene: 247814 Human
    • Unigene: 248176 Human
    • Unigene: 443021 Human
    • Unigene: 484990 Human
    • Unigene: 532144 Human
    • Unigene: 533292 Human
    • Unigene: 546315 Human
    • Unigene: 586261 Human
    • Unigene: 591778 Human
    • Unigene: 221301 Mouse
    • Unigene: 261657 Mouse
    • Unigene: 377874 Mouse
    • Unigene: 390558 Mouse
    • Unigene: 397328 Mouse
    • Unigene: 138090 Rat
    see all
  • Alternative names

    • H3 histone family member E pseudogene antibody
    • H3 histone family, member A antibody
    • H3/A antibody
    • H31_HUMAN antibody
    • H3F3 antibody
    • H3FA antibody
    • Hist1h3a antibody
    • HIST1H3B antibody
    • HIST1H3C antibody
    • HIST1H3D antibody
    • HIST1H3E antibody
    • HIST1H3F antibody
    • HIST1H3G antibody
    • HIST1H3H antibody
    • HIST1H3I antibody
    • HIST1H3J antibody
    • HIST3H3 antibody
    • histone 1, H3a antibody
    • Histone cluster 1, H3a antibody
    • Histone H3 3 pseudogene antibody
    • Histone H3.1 antibody
    • Histone H3/a antibody
    • Histone H3/b antibody
    • Histone H3/c antibody
    • Histone H3/d antibody
    • Histone H3/f antibody
    • Histone H3/h antibody
    • Histone H3/i antibody
    • Histone H3/j antibody
    • Histone H3/k antibody
    • Histone H3/l antibody
    see all

Images

  • Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
    Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
    All lanes : Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103) at 1 µg/ml

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

    Predicted band size: 15 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking buffer: 2% BSA

    Gel type: MES

  • Immunocytochemistry - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
    Immunocytochemistry - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)

    Immunocytochemistry analysis of ab5103 staining Histone H3 (citrulline R2 + R8 + R17) in MCF7 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab5103 at 1µg/mL and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 100% methanol (5 min).

  • Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
    Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
    All lanes : Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103) at 1 µg/ml

    Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) nuclear lysate
    Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) nuclear lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

    Predicted band size: 15 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking buffer: 2% BSA

    Gel type: MES

  • Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
    Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
    All lanes : Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103) at 0.2 µg/ml

    Lane 1 : HL60 whole cell lysate (negative control)
    Lane 2 : HL60 whole cell lysate + DMSO (solvent control)
    Lane 3 : HL60 whole cell lysate + DMSO + Calcium Ionophore (positive control)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti Rabbit IR680 at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?



    Loading Control: GAPDH

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab5103 overnight at 4°C. Antibody binding was detected using Goat anti Rabbit IR680 secondary at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

     

  • Peptide Array - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
    Peptide Array - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)

    All batches of ab5103 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - citrulline 2 + 8 + 17 peptide (ab32876), indicating that this antibody specifically recognises the Histone H3 - citrulline 2 + 8 + 17 modifications.

    ab32876 - Histone H3 - citrulline 2 + 8 + 17

    ab17566 - Histone H3 - unmodified

  • Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
    Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
    All lanes : Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103) at 1 µg/ml

    Lane 1 : HL60 (Human Caucasian promyelocytic leukaemia) DMSO and Calcium Ionophore treated Whole Cell Lysate with with 5% BSA
    Lane 2 : HL60 (Human Caucasian promyelocytic leukaemia) DMSO and Calcium Ionophore treated Whole Cell Lysate with with 5% milk
    Lane 3 : HL60 (Human Caucasian promyelocytic leukaemia) DMSO and Calcium Ionophore treated Whole Cell Lysate with with 3% milk

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above .

    Blots were developled with Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody

Protocols

  • ChIP protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (250)

    Publishing research using ab5103? Please let us know so that we can cite the reference in this datasheet.

    ab5103 has been referenced in 250 publications.

    • Jackson-Jones LH  et al. Stromal Cells Covering Omental Fat-Associated Lymphoid Clusters Trigger Formation of Neutrophil Aggregates to Capture Peritoneal Contaminants. Immunity 52:700-715.e6 (2020). PubMed: 32294409
    • Alhede M  et al. The origin of extracellular DNA in bacterial biofilm infections in vivo. Pathog Dis 78:N/A (2020). PubMed: 32196074
    • Song H  et al. HNF4A-AS1/hnRNPU/CTCF axis as a therapeutic target for aerobic glycolysis and neuroblastoma progression. J Hematol Oncol 13:24 (2020). PubMed: 32216806
    • de Bont CM  et al. Neutrophil proteases degrade autoepitopes of NET-associated proteins. Clin Exp Immunol 199:1-8 (2020). PubMed: 31661552
    • Chatterjee V  et al. Endothelial microvesicles carrying Src-rich cargo impair adherens junction integrity and cytoskeleton homeostasis. Cardiovasc Res 116:1525-1538 (2020). PubMed: 31504252
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 31 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Histone H3 (citrulline R2 + R8 + R17) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Mouse brain after stroke)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Mouse brain after stroke
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 10 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (citrulline R2 + R8 + R17) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (brain tissue)
    Permeabilization
    No
    Specification
    brain tissue
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Ran Xu

    Verified customer

    Submitted Feb 02 2021

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Histone H3 (citrulline R2 + R8 + R17) antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization
    Yes - 0.3% triton-PBS
    Specification
    Liver
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    正剛 田中 

    Verified customer

    Submitted Dec 17 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H3 (citrulline R2 + R8 + R17) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (pulmonary alveolar macrophages and epithelial cell)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH6 30min
    Permeabilization
    No
    Specification
    pulmonary alveolar macrophages and epithelial cell
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 1.5% · Temperature: 24°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 11 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (citrulline R2 + R8 + R17) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (mouse neutrophils)
    Permeabilization
    Yes - 0.2% Triton in PBS
    Specification
    mouse neutrophils
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Kai Sun

    Verified customer

    Submitted Nov 10 2020

    Western blot abreview for Anti-Histone H3 (citrulline R2 + R8 + R17) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (neutrophils)
    Gel Running Conditions
    Non-reduced Denaturing (10%)
    Loading amount
    20 µg
    Specification
    neutrophils
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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    Verified customer

    Submitted Aug 05 2020

    Western blot abreview for Anti-Histone H3 (citrulline R2 + R8 + R17) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (Vascular smooth muscle cell)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    Vascular smooth muscle cell
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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    Abcam user community

    Verified customer

    Submitted Dec 18 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (citrulline R2 + R8 + R17) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Vascular smooth muscle cell)
    Permeabilization
    Yes - NP40
    Specification
    Vascular smooth muscle cell
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
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    Abcam user community

    Verified customer

    Submitted Dec 18 2018

    Western blot abreview for Anti-Histone H3 (citrulline R2 + R8 + R17) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (RAW 264.7 cells)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    25 µg
    Specification
    RAW 264.7 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 07 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (citrulline R2 + R8 + R17) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (macrophage cell line)
    Permeabilization
    Yes - 0.1% Triton in PBS for 5 min
    Specification
    macrophage cell line
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 04 2018

    1-10 of 31 Abreviews or Q&A

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