Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Kidney H9 organoid)
Permeabilization
Yes - 0.05% Tween 20
Specification
Kidney H9 organoid
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Sea Block
Dilution
1/500
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
Anti-Histone H3 (di methyl K27) nuclear antibody signal is pseudo colored in Green. The negative nuclei appear in Blue using Hoechst. The antigen retrieval was performed with the NxGen decloaking chamber.
Alexa Fluor 647 conjugated secondary was used to avoid any background autofluorescence signal.
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Submitted Jan 24 2020