Histone H3 (di-methyl K27) Quantification Kit (Colorimetric) (ab115070)

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Overview

  • Product name
    Histone H3 (di-methyl K27) Quantification Kit (Colorimetric)
    See all Histone H3 (K27 methylation) kits
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay type
    Quantitative
  • Sensitivity
    2 ng/well
  • Range
    20 ng/well - 5000 ng/well
  • Assay time
    2h 30m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Mammals
  • Product overview

    Methylation of histone H3 at lysine 27 (K27) is a mark of gene inactivation and it is thought to mediate heterochromatin formation by forming a binding site for Polycomb. H3 (methyl K27) is also thought to have an important role in the repression of HOX genes during development and in X chromosome inactivation and imprinting. H3 (di-methyl K27), a modification enriched at pericentromic heterochromatin, is observed to be broadly distributed throughout all euchromatic sites and participates in silencing gene expression.

    Histone H3 (di-methyl K27) Quantification Kit (Colorimetric) (ab115070) allows the user to specifically measure global di-methylation of histone H3K27 colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

Properties

Images

  • Functional Studies - Histone H3 (di-methyl K27) Quantification Kit (Colorimetric) (ab115070)
    Functional Studies - Histone H3 (di-methyl K27) Quantification Kit (Colorimetric) (ab115070)

    ab115070 used to measure the amount of di-methyl H3K27. Histone extracts were prepared from MDA-MB-231 cells using the Histone Extraction Kit (ab113476).

Protocols

References

ab115070 has not yet been referenced specifically in any publications.

Publishing research using ab115070? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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4 reviews or Q&A

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The credit was about ******* and this time, he has ******.
These kits were not satisfied again, he already purchased other vendor's products and doesn't need to use ab115070, ab115072 any more.
Please arrange credit note for our customer.

Sorry for the confusion.

As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order.

Credit ID: *****

As usual if you have any fu...

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Please arrange credit note for our customer.

A credit note has already been issued on the DD MM YYYY for these two kits. The reference of the credit note is ********.

In my previous email I said : "Would your customer agree to use their credit note to buy and try the kits for the 3...

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As you know our customer is is havinga problem using you ab115070, ab115072.
He recently received new kits and tested again, but he could not any results eventhoug at standard.
So today I met him and checked his protocols, and there was no pr...

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Please find below the last email I received from the lab where the kits are produced :

"We are sorry to hear about it and not clear what caused it not work.
The procedure seems fine.
We did not receive any other complaint for t...

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As you know our customer is is havinga problem using you ab115070, ab115072.
He recently received new kits and tested again, but he could not any results eventhoug at standard.
So today I met him and checked his protocols, and there was no pr...

Read More

Thank you for contacting us.

Thank you also for the detailed information about the use of the kits ab115070 and ab115072.

I do believe there is a problem with those kits, so please send our sincere apologies to the customer.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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