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Is this antibody specific to Histone H3 (di methyl K4)?
Asked on Dec 10 2012
For ab32356 (https://www.abcam.com/histone-h3-di-methyl-k4-antibody-y47-chip-grade-ab32356.html):
Based on our western blot testing testing, ab32356 should specifically detect Histone H3 dimethylated on Lysine 4. The WB figure on the product datasheet shows that no signal was observed on non-methylated recombinant Histone H3 (˜1ug).
Recommended amount: Use 8ul for 25ug of chromatin
For ab7766 (https://www.abcam.com/histone-h3-di-methyl-k4-antibody-chip-grade-ab7766.html):
All batches of ab7766 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - di methyl K4 peptide (ab7768), indicating that this antibody specifically recognizes the Histone H3 - di methyl K4 modification. I've attached the results of one of these peptide arrays to this email.
Recommended amount: Use 2ug for 25ug of chromatin
Answered on Dec 10 2012