Western blot abreview for Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Monkey Cell lysate - whole cell (COS1)
Loading amount
20 µg
Specification
COS1
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 17 kDa Non-specific: 70 kDa

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Submitted Nov 05 2012

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