Immunohistochemistry (Frozen sections) abreview for Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse bronchiolar epithelium)
Specification
Mouse bronchiolar epithelium
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 27°C

Other product details

Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 27°C

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

Additional data

Additional Notes
Requires heat antigen retrieval in citrate buffer. Most of the nuclear markers often require antigen retrieval even though they are frozen sections.

Abcam user community

Verified customer

Submitted Nov 15 2012

Sign up