Western blot abreview for Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade

Good
Abreviews
Application
Western blot
Sample
Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Whole fly tissue)
Gel Running Conditions
Reduced Denaturing (gradient precasted gel, Biorad 567-8124)
Loading amount
40 µg
Specification
Whole fly tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA TBST
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: Cell signalling secondary 7074S
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 17 kDa Non-specific: yes kDa

Additional data

Additional Notes
Whole fly samples were boiled in Laemmeli sample buffer + DTT and then loaded on a precasted Biorad gel (567-8124 Criterion gel). TBST buffer used. Several non specific bands observed.

Abcam user community

Verified customer

Submitted May 06 2016

Sign up