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Epigenetics and Nuclear Signaling Histones H3 Methylated
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Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594)

  • Datasheet
  • SDS
Reviews (13)Q&A (1)References (173)

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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594)
  • ChIP - Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594)
  • Western blot - Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594)
  • Peptide Array - Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594)
  • Western blot - Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594)
  • ChIP - Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594)

Key features and details

  • Rabbit polyclonal to Histone H3 (di methyl K79) - ChIP Grade
  • Suitable for: ChIP, WB, ICC/IF, IHC-P, PepArr
  • Reacts with: Cow, Human
  • Isotype: IgG

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Anti-TIM 3 antibody [EPR22241] - BSA and Azide free (ab242080)

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Overview

  • Product name

    Anti-Histone H3 (di methyl K79) antibody - ChIP Grade
    See all Histone H3 primary antibodies
  • Description

    Rabbit polyclonal to Histone H3 (di methyl K79) - ChIP Grade
  • Host species

    Rabbit
  • Specificity

    ab3594 detects a 17 kDa band in single lane Western Blot. Peptide inhibition in Western Blot hasn't been processed. Modification specificity is determined by Peptide Array. ab3594 binds strongly to the Histone H3 dii methyl K79. In Peptide Array ab3594 also partially binds to mono methyl K79 and tri methyl K79 peptides.
  • Tested Applications & Species

    Application Species
    ChIP
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Cow
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Histone H3 aa 50 to the C-terminus (di methyl K79) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    (Peptide available as ab4556)

  • General notes

    Learn about ChIP assay kits, other ChIP antibodies, protocols and more in the ChIP assay guide.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Methylated
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies

Associated products

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Control Peptide

    • Human Histone H3 (di methyl K9) peptide (ab1772)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Histone H3 protein (Active) (ab198757)
  • Related Products

    • Histone H3 (di-methyl K79) Quantification Kit (Colorimetric) (ab115085)
    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ChIP
Human
ICC/IF
Human
IHC-P
Human
WB
Cow
All applications
Mouse
Pig
Saccharomyces cerevisiae
Caenorhabditis elegans
a wide range of other species
Mammals
Silk worm
Triticum aestivum
Application Abreviews Notes
ChIP (2)
Use at an assay dependent concentration.
WB (6)
Use at an assay dependent concentration. Predicted molecular weight: 15 kDa.
ICC/IF (3)
Use a concentration of 1 µg/ml.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
PepArr
Use a concentration of 0.2 - 0.02 µg/ml.
Notes
ChIP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 15 kDa.
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
PepArr
Use a concentration of 0.2 - 0.02 µg/ml.

Target

  • Function

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities

    Belongs to the histone H3 family.
  • Developmental stage

    Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
  • Post-translational
    modifications

    Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
    Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
    Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
    Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
    Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
    Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
  • Cellular localization

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P68431 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8350 Human
    • Entrez Gene: 8351 Human
    • Entrez Gene: 8352 Human
    • Entrez Gene: 8353 Human
    • Entrez Gene: 8354 Human
    • Entrez Gene: 8355 Human
    • Entrez Gene: 8356 Human
    • Entrez Gene: 8357 Human
    • Entrez Gene: 8358 Human
    • Entrez Gene: 8968 Human
    • Entrez Gene: 319152 Mouse
    • Entrez Gene: 319153 Mouse
    • Entrez Gene: 360198 Mouse
    • Entrez Gene: 97908 Mouse
    • Omim: 602810 Human
    • SwissProt: P68432 Cow
    • SwissProt: P68431 Human
    • SwissProt: P68433 Mouse
    • Unigene: 132854 Human
    • Unigene: 247813 Human
    • Unigene: 247814 Human
    • Unigene: 248176 Human
    • Unigene: 443021 Human
    • Unigene: 484990 Human
    • Unigene: 532144 Human
    • Unigene: 533292 Human
    • Unigene: 546315 Human
    • Unigene: 586261 Human
    • Unigene: 591778 Human
    • Unigene: 221301 Mouse
    • Unigene: 261657 Mouse
    • Unigene: 377874 Mouse
    • Unigene: 390558 Mouse
    • Unigene: 397328 Mouse
    see all
  • Alternative names

    • H3 histone family member E pseudogene antibody
    • H3 histone family, member A antibody
    • H3/A antibody
    • H31_HUMAN antibody
    • H3F3 antibody
    • H3FA antibody
    • Hist1h3a antibody
    • HIST1H3B antibody
    • HIST1H3C antibody
    • HIST1H3D antibody
    • HIST1H3E antibody
    • HIST1H3F antibody
    • HIST1H3G antibody
    • HIST1H3H antibody
    • HIST1H3I antibody
    • HIST1H3J antibody
    • HIST3H3 antibody
    • histone 1, H3a antibody
    • Histone cluster 1, H3a antibody
    • Histone H3 3 pseudogene antibody
    • Histone H3.1 antibody
    • Histone H3/a antibody
    • Histone H3/b antibody
    • Histone H3/c antibody
    • Histone H3/d antibody
    • Histone H3/f antibody
    • Histone H3/h antibody
    • Histone H3/i antibody
    • Histone H3/j antibody
    • Histone H3/k antibody
    • Histone H3/l antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594)Tao J. et al PLoS One. 2017 Jun 20;12(6):e0179436. doi: 10.1371/journal.pone.0179436. eCollection 2017. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
    Verification of H3K79me2 antibody specificity.

    The commercial H3K79me2 primary antibody was preincubated with (+) or without (−) antigen peptide (Abcam, catalog no. ab4556, v/v = 5:1) at room temperature for 1.5 h before the incubation with IVF porcine blastocysts. H3K79me2 signals were observed in blastocysts using unabsorbed primary antibody. By contrast, H3K79me2 signals were absent in blastocysts using pre-absorbed primary antibody. H3K79me2 antibody was localized with Alexa Flour 488-conjugated secondary antibody (green). DNA was stained with propidium iodide (red). Bottom panels showed the merged images (yellow) between H3K79me2 signals (green) and DNA staining (red). Scale bars = 50μm.

  • ChIP - Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594)
    ChIP - Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594)

    Chromatin was prepared from U-2 OS (Human bone osteosarcoma epithelial cell line) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The  ChIP was performed with 25 µg of chromatin, 2 µg of  ab3594 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

  • Western blot - Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594)
    Western blot - Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594)This image is courtesy of an anonymous Abreview
    Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594) at 20 µg + HeLa cell lysate at 20 µg

    Secondary
    HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 15 kDa


    Exposure time: 1 minute

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594)

    ICC/IF image of ab3594 stained HeLa (Human epithelial adenocarcinoma cell line) cells. The cells were 100% methanol fixed (5 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3594, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 1µg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594)

    IHC image of Histone H3 (di methyl K79) staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3594, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • Peptide Array - Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594)
    Peptide Array - Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594)

    All batches of ab3594 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - di methyl K79 peptide (ab4556), indicating that this antibody specifically recognises the Histone H3 - di methyl K79 modification.

    ab4556 - Histone H3 - di methyl K79

    ab4555 - Histone H3 - mono methyl K79

    ab4557 - Histone H3 - tri methyl K79

    ab4560 - Histone H4 - di methyl K79

    ab1772 - Histone H3 - di methyl K9

    ab4558 - Histone H3 - unmodified

  • Western blot - Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594)
    Western blot - Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594)
    Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594) at 1 µg/ml + Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds
  • ChIP - Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594)
    ChIP - Anti-Histone H3 (di methyl K79) antibody - ChIP Grade (ab3594)This image is courtesy of an anonymous Abreview

    ChIP analysis using ab3594 binding Histone H3 in HeLa (Human epithelial adenocarcinoma cell line) cell nuclear lysate. Cells were cross-linked for 10 minutes with formaldehyde. Samples were incubated with primary antibody (5µg/µg chromatin) for 12 hours at 4°C. Protein binding was detected using real-time PCR.
    Positive control: 5'UTR of transcribed gene.
    Negative Control: Intergenic region.

    See Abreview

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (173)

    Publishing research using ab3594? Please let us know so that we can cite the reference in this datasheet.

    ab3594 has been referenced in 173 publications.

    • Yoo H  et al. Epigenetic priming by Dot1l in lymphatic endothelial progenitors ensures normal lymphatic development and function. Cell Death Dis 11:14 (2020). PubMed: 31908356
    • Tatehana M  et al. Comprehensive histochemical profiles of histone modification in male germline cells during meiosis and spermiogenesis: Comparison of young and aged testes in mice. PLoS One 15:e0230930 (2020). PubMed: 32267870
    • Liu C  et al. CBP mediated DOT1L acetylation confers DOT1L stability and promotes cancer metastasis. Theranostics 10:1758-1776 (2020). PubMed: 32042335
    • Szewczyk MM  et al. Pharmacological inhibition of PRMT7 links arginine monomethylation to the cellular stress response. Nat Commun 11:2396 (2020). PubMed: 32409666
    • So CC  et al. E3 Ubiquitin Ligases RNF20 and RNF40 Are Required for Double-Stranded Break (DSB) Repair: Evidence for Monoubiquitination of Histone H2B Lysine 120 as a Novel Axis of DSB Signaling and Repair. Mol Cell Biol 39:N/A (2019). PubMed: 30692271
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 14 Abreviews or Q&A

    ELISA abreview for Anti-Histone H3 (di methyl K79) antibody - ChIP Grade

    Poor
    Abreviews
    Abreviews
    Application
    ELISA
    Sample
    Human Purified protein (PBCMs)
    Specification
    PBCMs
    Type
    Sandwich (Capture)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3%
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 06 2014

    Western blot abreview for Anti-Histone H3 (di methyl K79) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (12)
    Sample
    Human Cell lysate - whole cell (Human Hela cells)
    Specification
    Human Hela cells
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 18 2013

    ChIP abreview for Anti-Histone H3 (methyl (di) K79) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Detection step
    Real-time PCR
    Sample
    Human Cell lysate - nuclear (HeLa)
    Specification
    HeLa
    Negative control
    Intergenic region
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde
    Positive control
    5'UTR of a transcribed gene
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 01 2013

    Western blot abreview for Anti-Histone H3 (di methyl K79) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Caenorhabditis elegans Tissue lysate - nuclear (C. elegans)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    10 µg
    Treatment
    0, 75Gy r-rays
    Specification
    C. elegans
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Ms. Jinsun Ryu

    Verified customer

    Submitted Sep 12 2012

    Western blot abreview for Anti-Histone H3 (methylated) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (Liver)
    Loading amount
    20 µg
    Specification
    Liver
    Treatment
    X protein was knockdowned
    Gel Running Conditions
    Reduced Denaturing (15)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 22 2011

    ChIP abreview for Anti-Histone H3 (methylated) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (K562)
    Specification
    K562
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% formaldehyde
    Detection step
    Other
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 01 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (methylated) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (293 cells)
    Specification
    293 cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 1% SDS
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
    Read More

    Dr. Kathrin Bernt

    Verified customer

    Submitted Dec 11 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (methylated) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (10T1/2)
    Specification
    10T1/2
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Sep 12 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (methylated) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Sep 12 2008

    Immunoprecipitation abreview for Anti-Histone H3 (di methyl K79) antibody - ChIP Grade

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Caenorhabditis elegans Cell lysate - whole cell (Embryo)
    Total protein in input
    250 µg
    Immuno-precipitation step
    Protein A
    Specification
    Embryo
    Read More

    Dr. Caroline Thivierge

    Verified customer

    Submitted Jan 25 2008

    1-10 of 14 Abreviews or Q&A

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