Histone H3 (di-methyl K9) Quantification Kit (Fluorometric) (ab115063)

Overview

  • Product name
    Histone H3 (di-methyl K9) Quantification Kit (Fluorometric)
    See all Histone H3 (K9 methylation) kits
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay type
    Quantitative
  • Assay time
    2h 30m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Mammals
  • Product overview

    Methylation of histone H3 at lysine 9 by ESET, G9a among other methyl transferases is involved in euchromatin gene silencing as well as in heterochromatin formation. Specifically, H3 (di-methyl K9) mediates heterochromatin formation by forming a binding site for HP1.

    Histone H3 (di-methyl K9) Quantification Kit (Fluorometric) (ab115063) allows the user to specifically measure global di-methylation of histone H3K9 fluorometrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.

Properties

Images

  • Histone extracts were prepared from MDA-MB-231 cells using the Histone Extraction Kit (ab113476) and the amount of H3K9me2 was measured using ab115063.

Protocols

References

ab115063 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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I have heard back from the lab and they said that 1% SDS in the lysis buffer used for protein extractionshould not cause problems with use of this kit.

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