Key features and details
- Assay type: Quantitative
- Platform: Microplate reader
- Assay time: 3 hr
- Sample type: Cell Lysate, Tissue Extracts
Product nameHistone H3 (K4) Methyltransferase Activity Quantification Assay Kit
See all Histone Methyltransferase kits
Sample typeTissue Extracts, Cell Lysate
Assay time3h 00m
Species reactivityReacts with: Mouse, Human
Predicted to work with: Mammals
Methylation of histone H3 at lysine 4 (K4) is thought to be a global epigenetic mark in euchromatin and a mediator of activated transcription. In mammalian cells, several SET-domain containing histone lysine methyltransferases such as MLL1 are responsible for adding one, two or three methyl groups at lysine 4 of histone H3.
Histone H3 (K4) Methyltransferase Activity Quantification Assay Kit (ab113452) allows the user to measure the activity or inhibition of individual histone methyltransferases that specifically target histone H3 at lysine 4 (H3K4). The kit is ready-to-use and provides all the essential components needed to carry out a successful histone methyltransferase activity/inhibition quantification assay without the need for radioactivity or any special equipment.
Storage instructionsPlease refer to protocols.
Components 48 tests 96 tests 10X Wash Buffer 1 x 11ml 1 x 22ml 8-Well Assay Strips (with Frame) 6 units 12 units Adomet 1 x 25µl 1 x 50µl Biotinylated Substrate, 25 µg/mL 1 x 100µl 1 x 200µl Capture Antibody, 100 µg/mL 1 x 25µl 1 x 50µl Control Enzyme (200 µg/mL) 1 x 5µl 1 x 10µl Detection Antibody, 200 µg/mL 1 x 10µl 1 x 20µl Developing Solution 1 x 6ml 1 x 12ml Histone Assay Buffer 1 x 1.5ml 1 x 3ml HMT Standard, 10 µg/mL 1 x 10µl 1 x 20µl Stop Solution 1 x 3ml 1 x 6ml
Nuclear extracts were prepared from MCF-7 cells using the ab113474 and H3-K4 specific histone methyltrasferase activity (SET7/9) was measured.
ab113452 has not yet been referenced specifically in any publications.