Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit (ab113453)

Abreviews (1)Q&A (5)

Overview

  • Product name
    Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit
    See all Histone Methyltransferase kits
  • Assay type
    Quantitative
  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Mammals
  • Product overview

    Methylation of histone H3 at lysine 9 (K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing expression at euchromatic sites. In mammalian cells, histone lysine methyltransferases such as SuVar39h and ESET among others are responsible for methylation of lysine 9 at histone H3.

    Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit (ab113453) allows the user to measure the activity or inhibition of individual histone methyltransferase (HMT) that specifically target histone H3 at lysine 9 (H3K9). The kit is ready-to-use and provides all the essential components needed to carry out a successful HMT activity/inhibition quantification experiment without the need for radioactivity or any special equipment.

    ab113453 recognizes methylated H3 at lysine site 9.

Properties

Images

  • Functional Studies - Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit (ab113453)
    Functional Studies - Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit (ab113453)
    Nuclear extracts were prepared from MCF-7 cells using the ab113474 and H3-K9 specific histone methyltrasferase activity (G9a) was measured. Purified G9a was spiked into the nuclear extract that has low concentration of nuclear proteins by dilution.

Protocols

References

ab113453 has not yet been referenced specifically in any publications.

Publishing research using ab113453? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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6 reviews or Q&A

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Questions to assay principle and componens:

1. Is the substrate a peptide or protein fragment?
2. Why is the substrate biotinylated? Is the plate avidin-coated? Or is other coating material used?
3. Which methylation is being detec...

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Thank you for contacting us.
Here are the answers to your questions:
1. Peptide.
2. Yes, avidin-coated.
3. This information cannot be disclosed currently.
4. No. unless a purified enzyme or extract with the over-expressed speci...

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Standard Curve (plot of OD value versus amount of HMT Standard) for H3K9 Methyltransferase Activity Quantification Assay Kit.

 Excellent Good 4/5 (Ease of Use)
Abreviews
A standard curve was plotted by plotting the blank corrected OD450 for three different HMT standard concentrations. This kit works well and is specific for H3K9 Me-transferase.
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Submitted Mar 10 2017

1.Can any of the components be stored when diluted or must they be used immediately?
2. At what temperature should diluted components be stored?
3. If the detection antibody is conjugated, why should it be kept frozen?

Thank you for contacting us.
Here are the answers to your questions:
1. After dilution, each diluted component should be used within the same day.
2. At 4C for same day.
3. for longer time storage, as it will not be frozen at -20C. ...

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1. How many cells or mg of tissue will yield 4-20ug of nuclear extract?
2. Can customer perform this assay using their own 96-well plate? Maybe after avidin coating?
3. The protocol says to read the absorbance within 2-15 minutes? Would it be...

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Thank you for contacting us.
Here are the answers to your questions:
1. 100,000-500,000 cells or 10-50 mg tissues.
2. It is possible but results are not guaranteed.
3. It can be stable for 30 min after adding stop solution.
4. ...

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wants to use rat oligodendrocytes with and without inhibitors for MT
1) is the kit suitable for these conditions?
2) image on datasheet: was done specifically done for G9a. How was it specifically measured for this methyltransferase while exc...

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Thank you for contacting us and thank you for your patience.

I have heard back from the lab with the following information:

1. Yes, this kit is suitable for the conditions mentioned (rat oligodendrocytes with and without inhibitors...

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I am interested in using this kit to study methyltransferase activity in a species of fly. Do you know if anyone has successfully used it with Drosophila? Thanks.

Thanks for your inquiry. Yes, the kit ab113453 is suitable for measuring H3K9 activity from various species including Drosophila based on the working principle and antibody's specificity. Please let me know if you require further ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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