Overview

  • Product name
    Histone H3 (methyl K4) Assay Kit (In Situ)
    See all Histone H3 (K4 methylation) kits
  • Sample type
    Adherent cells
  • Assay type
    Cell-based (quantitative)
  • Assay time
    3h 00m
  • Product overview

    Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions and may serve as a global epigenetic mark in euchromatin. H3 (methyl K4) also appears to have an inverse correlation to allele-specific DNA methylation.

    Histone H3 (methyl K4) Assay Kit (In Situ) (ab115046) allows the user to specifically measure histone H3K4 methylation in situ using cultured adherent cells in a quick and efficient procedure which can be finished within 3 hours.

Properties

  • Storage instructions
    Please refer to protocols.
  • Components 96 tests 2 x 96 tests
    10X Wash Buffer 1 x 30ml 2 x 30ml
    30% H2O2 Solution 1 x 0.5ml 1 x 1ml
    8-Well Control Strips 2 units 4 units
    Antibody Buffer 1 x 10ml 1 x 20ml
    Blocking Buffer 1 x 20ml 2 x 20ml
    Capture Antibody, 100 µg/mL 1 x 60µl 1 x 120µl
    Detection Antibody, 200 µg/mL 1 x 20µl 1 x 40µl
    Developing Solution 1 x 12ml 1 x 24ml
    Methylated H3K4 Control, 20 µg/mL 1 x 15µl 1 x 30µl
    Microplates 1 unit 2 units
    Permeabilizing Buffer 1 x 30ml 2 x 30ml
    Stop Solution 1 x 6ml 1 x 12ml
  • Research areas

Protocols

References

ab115046 has not yet been referenced specifically in any publications.

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