Histone H3 Modification Multiplex Assay Kit (Colorimetric) (ab185910)

Reviews (1) Submit a question References (1)
Typical data

    Key features and details

    • Assay type: Quantitative
    • Detection method: Colorimetric
    • Platform: Microplate reader
    • Assay time: 2 hr 30 min
    • Sample type: Adherent cells, Suspension cells, Tissue
    • Sensitivity: 0.5 ng/well

    Overview

    • Product name

      Histone H3 Modification Multiplex Assay Kit (Colorimetric)

    • Detection method

      Colorimetric

    • Sample type

      Tissue, Adherent cells, Suspension cells

    • Assay type

      Quantitative

    • Sensitivity

      0.5 ng/well

    • Range

      20 ng/well - 500 ng/well

    • Assay time

      2h 30m

    • Species reactivity

      Reacts with: Mouse, Rat, Human

    • Product overview

      The Histone H3 Modification Multiplex Assay Kit (Colorimetric) (ab185910) is a complete set of optimized reagents to detect and quantify up to 21 modified Histone H3 patterns simultaneously in a simple, ELISA-like format with use of a standard microplate reader. 

    • Platform

      Microplate reader

    Properties

    Associated products

    Images

    • Typical data
      Typical data

      Histone extracts were prepared from MCF-7 and MDA-231 cells and multiple Histone H3 modifications were screened and measured using the Histone H3 Modification Multiplex Assay Kit (Colorimetric). 100 ng of total histone proteins were used.

    References (1)

    Publishing research using ab185910? Please let us know so that we can cite the reference in this datasheet.

    ab185910 has been referenced in 1 publication.

    • Lillico R  et al. HDAC inhibitors induce global changes in histone lysine and arginine methylation and alter expression of lysine demethylases. J Proteomics 133:125-33 (2016). PubMed: 26721445

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Testing Histone H3 modification multiplex assay kit (colorimetric) on bovine samples

     Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    We used this product to detect histone modifications on bovine granulosa cells. It worked really well and gave good
    signal. The background was very low even with brief washings.

    Protocol:
    1. Histone from bovine granulosa cells were extracted using histone extraction kit (ab113476)
    2. Protein concentration in lysate was then measured using Bradford assay (BioRad)
    3. 50 ng of histone extract was used per well in H3 modification multiplex assay kit (ab185910)

    Abcam user community

    Verified customer

    Submitted Dec 08 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com