For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    histone-h3-modification-multiplex-assay-kit-colorimetric-ab185910.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Histones H3 Unmodified
Share by email

Histone H3 Modification Multiplex Assay Kit (Colorimetric) (ab185910)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (1) Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Typical data

    Key features and details

    • Assay type: Quantitative
    • Detection method: Colorimetric
    • Platform: Microplate reader
    • Assay time: 2 hr 30 min
    • Sample type: Adherent cells, Suspension cells, Tissue
    • Sensitivity: 0.5 ng/well

    You may also be interested in

    Preparation
    Product image
    Histone Extraction Kit (ab113476)
    Biochemical
    GSK-J1, H3K27 histone demethylase inhibitor (ab144396)
    ELISA
    Product image
    Human SIRT1 ELISA Kit (ab171573)

    View more associated products

    Overview

    • Product name

      Histone H3 Modification Multiplex Assay Kit (Colorimetric)
    • Detection method

      Colorimetric
    • Sample type

      Tissue, Adherent cells, Suspension cells
    • Assay type

      Quantitative
    • Sensitivity

      0.5 ng/well
    • Range

      20 ng/well - 500 ng/well
    • Assay time

      2h 30m
    • Species reactivity

      Reacts with: Mouse, Rat, Human
    • Product overview

      The Histone H3 Modification Multiplex Assay Kit (Colorimetric) (ab185910) is a complete set of optimized reagents to detect and quantify up to 21 modified Histone H3 patterns simultaneously in a simple, ELISA-like format with use of a standard microplate reader. 

    • Platform

      Microplate reader

    Properties

    • Storage instructions

      Please refer to protocols.
    • Components 96 tests
      10X Wash Buffer 1 x 28ml
      8-Well Strips 2 units
      96-Well Strip Plate (with Frame) 1 unit
      Adhesive Covering Film 1 unit
      Antibody Buffer 1 x 8ml
      Assay Control Protein 1 x 20µl
      Detection Antibody 1 x 12µl
      Developer Solution 1 x 12ml
      Stop Solution 1 x 12ml
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Histones
      • H3
      • Unmodified
      • Epigenetics and Nuclear Signaling
      • Histones
      • H3
      • Methylated
      • Epigenetics and Nuclear Signaling
      • Histones
      • H3
      • Ubiquitinated
      • Epigenetics and Nuclear Signaling
      • Histones
      • H3
      • Acetylated
      • Epigenetics and Nuclear Signaling
      • Histones
      • H3
      • Phosphorylated
      • Kits/ Lysates/ Other
      • Kits
      • Epigenetic kits
      • DNA modification and methylation

    Associated products

    • Related Products

      • Histone Extraction Kit (ab113476)

    Images

    • Typical data
      Typical data

      Histone extracts were prepared from MCF-7 and MDA-231 cells and multiple Histone H3 modifications were screened and measured using the Histone H3 Modification Multiplex Assay Kit (Colorimetric). 100 ng of total histone proteins were used.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab185910? Please let us know so that we can cite the reference in this datasheet.

    ab185910 has been referenced in 1 publication.

    • Lillico R  et al. HDAC inhibitors induce global changes in histone lysine and arginine methylation and alter expression of lysine demethylases. J Proteomics 133:125-33 (2016). PubMed: 26721445

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Testing Histone H3 modification multiplex assay kit (colorimetric) on bovine samples

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    We used this product to detect histone modifications on bovine granulosa cells. It worked really well and gave good
    signal. The background was very low even with brief washings.

    Protocol:
    1. Histone from bovine granulosa cells were extracted using histone extraction kit (ab113476)
    2. Protein concentration in lysate was then measured using Bradford assay (BioRad)
    3. 50 ng of histone extract was used per well in H3 modification multiplex assay kit (ab185910)

    Abcam user community

    Verified customer

    Submitted Dec 08 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.