Histone H3 Modification Multiplex Assay Kit (Colorimetric) (ab185910)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameHistone H3 Modification Multiplex Assay Kit (Colorimetric)
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Detection methodColorimetric
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Sample typeTissue, Adherent cells, Suspension cells
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Assay typeQuantitative
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Sensitivity0.5 ng/well
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Range20 ng/well - 500 ng/well
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Assay time2h 30m
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Species reactivityReacts with: Mouse, Rat, Human, Plants, Fungi, Bacteria
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Product overview
The Histone H3 Modification Multiplex Assay Kit (Colorimetric) (ab185910) is a complete set of optimized reagents to detect and quantify up to 21 modified Histone H3 patterns simultaneously in a simple, ELISA-like format with use of a standard microplate reader.
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PlatformMicroplate reader
Properties
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Storage instructionsPlease refer to protocols.
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Components 96 tests 10X Wash Buffer 1 x 28ml 8-Well Strips 2 units 96-Well Strip Plate (with Frame) 1 unit Adhesive Covering Film 1 unit Antibody Buffer 1 x 8ml Assay Control Protein 1 x 20µl Detection Antibody 1 x 12µl Developer Solution 1 x 12ml Stop Solution 1 x 12ml -
Research areas
Associated products
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Related Products
Images
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Typical dataHistone extracts were prepared from MCF-7 and MDA-231 cells and multiple Histone H3 modifications were screened and measured using the Histone H3 Modification Multiplex Assay Kit (Colorimetric). 100 ng of total histone proteins were used.
Protocols
References
This product has been referenced in:
- Lillico R et al. HDAC inhibitors induce global changes in histone lysine and arginine methylation and alter expression of lysine demethylases. J Proteomics 133:125-33 (2016). Read more (PubMed: 26721445) »
