Histone H3 Modification Multiplex Assay Kit (Colorimetric) (ab185910)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 2 hr 30 min
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 0.5 ng/well
Overview
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Product name
Histone H3 Modification Multiplex Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Quantitative -
Sensitivity
0.5 ng/well -
Range
20 ng/well - 500 ng/well -
Assay time
2h 30m -
Species reactivity
Reacts with: Mouse, Rat, Human -
Product overview
The Histone H3 Modification Multiplex Assay Kit (Colorimetric) (ab185910) is a complete set of optimized reagents to detect and quantify up to 21 modified Histone H3 patterns simultaneously in a simple, ELISA-like format with use of a standard microplate reader.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 96 tests 10X Wash Buffer 1 x 28ml 8-Well Strips 2 units 96-Well Strip Plate (with Frame) 1 unit Adhesive Covering Film 1 unit Antibody Buffer 1 x 8ml Assay Control Protein 1 x 20µl Detection Antibody 1 x 12µl Developer Solution 1 x 12ml Stop Solution 1 x 12ml -
Research areas
Associated products
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Related Products
Images
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Typical data
Histone extracts were prepared from MCF-7 and MDA-231 cells and multiple Histone H3 modifications were screened and measured using the Histone H3 Modification Multiplex Assay Kit (Colorimetric). 100 ng of total histone proteins were used.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab185910 has been referenced in 1 publication.
- Lillico R et al. HDAC inhibitors induce global changes in histone lysine and arginine methylation and alter expression of lysine demethylases. J Proteomics 133:125-33 (2016). PubMed: 26721445