Histone H3 Modification Multiplex Assay Kit (Colorimetric) (ab185910)

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Overview

  • Product name
    Histone H3 Modification Multiplex Assay Kit (Colorimetric)
    See all Histone H3 kits
  • Detection method
    Colorimetric
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay type
    Quantitative
  • Sensitivity
    0.5 ng/well
  • Range
    20 ng/well - 500 ng/well
  • Assay time
    2h 30m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Plants, Fungi, Bacteria
  • Product overview

    The Histone H3 Modification Multiplex Assay Kit (Colorimetric) (ab185910) is a complete set of optimized reagents to detect and quantify up to 21 modified Histone H3 patterns simultaneously in a simple, ELISA-like format with use of a standard microplate reader. 

  • Platform
    Microplate

Properties

Associated products

Images

  • Typical data
    Typical data

    Histone extracts were prepared from MCF-7 and MDA-231 cells and multiple Histone H3 modifications were screened and measured using the Histone H3 Modification Multiplex Assay Kit (Colorimetric). 100 ng of total histone proteins were used.

Protocols

References

This product has been referenced in:
  • Lillico R  et al. HDAC inhibitors induce global changes in histone lysine and arginine methylation and alter expression of lysine demethylases. J Proteomics 133:125-33 (2016). Read more (PubMed: 26721445) »

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Customer reviews and Q&As

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1 Review or Q&A

10
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Testing Histone H3 modification multiplex assay kit (colorimetric) on bovine samples

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
We used this product to detect histone modifications on bovine granulosa cells. It worked really well and gave good
signal. The background was very low even with brief washings.

Protocol:
1. Histone from bovine granulosa cells were extracted using histone extraction kit (ab113476)
2. Protein concentration in lysate was then measured using Bradford assay (BioRad)
3. 50 ng of histone extract was used per well in H3 modification multiplex assay kit (ab185910)
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Submitted Dec 08 2015

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