Histone H3 Modification Multiplex Assay Kit (Colorimetric) (ab185910)

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Overview

  • Product name

    Histone H3 Modification Multiplex Assay Kit (Colorimetric)

  • Detection method

    Colorimetric

  • Sample type

    Tissue, Adherent cells, Suspension cells

  • Assay type

    Quantitative

  • Sensitivity

    0.5 ng/well

  • Range

    20 ng/well - 500 ng/well

  • Assay time

    2h 30m

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Product overview

    The Histone H3 Modification Multiplex Assay Kit (Colorimetric) (ab185910) is a complete set of optimized reagents to detect and quantify up to 21 modified Histone H3 patterns simultaneously in a simple, ELISA-like format with use of a standard microplate reader. 

  • Platform

    Microplate reader

Properties

Associated products

Images

  • Typical data
    Typical data

    Histone extracts were prepared from MCF-7 and MDA-231 cells and multiple Histone H3 modifications were screened and measured using the Histone H3 Modification Multiplex Assay Kit (Colorimetric). 100 ng of total histone proteins were used.

Protocols

References

This product has been referenced in:

  • Lillico R  et al. HDAC inhibitors induce global changes in histone lysine and arginine methylation and alter expression of lysine demethylases. J Proteomics 133:125-33 (2016). Read more (PubMed: 26721445) »
See 1 Publication for this product

Publishing research using ab185910? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Testing Histone H3 modification multiplex assay kit (colorimetric) on bovine samples

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
We used this product to detect histone modifications on bovine granulosa cells. It worked really well and gave good
signal. The background was very low even with brief washings.

Protocol:
1. Histone from bovine granulosa cells were extracted using histone extraction kit (ab113476)
2. Protein concentration in lysate was then measured using Bradford assay (BioRad)
3. 50 ng of histone extract was used per well in H3 modification multiplex assay kit (ab185910)

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Verified customer

Submitted Dec 08 2015

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