Western blot abreview for Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade

Good
Abreviews
Application
Western blot
Sample
Human Recombinant protein (Peptides)
Gel Running Conditions
Reduced Denaturing
Loading amount
0.4 µg
Specification
Peptides
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Milk
Dilution
1/5000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)

Additional data

Additional Notes
The antibody worked very well for dot blot assays as there was no cross-reactivity with other peptides.

Ifeoluwa Adewumi

Verified customer

Submitted Feb 28 2017

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