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Epigenetics and Nuclear Signaling Histones H3 Methylated
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Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)

  • Datasheet
Reviews (10)Q&A (2)References (47)

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ChIP - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
  • Western blot - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
  • Peptide Array - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
  • Western blot - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
  • ChIP - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
  • Immunoprecipitation - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)

Key features and details

  • Rabbit polyclonal to Histone H3 (mono methyl K79) - ChIP Grade
  • Suitable for: ChIP, IP, WB, PepArr
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG

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Primary
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Protein
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Recombinant human Histone H3 protein (Active) (ab198757)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade
    See all Histone H3 primary antibodies
  • Description

    Rabbit polyclonal to Histone H3 (mono methyl K79) - ChIP Grade
  • Host species

    Rabbit
  • Specificity

    Reacts with Mono-methyl K79 of histone H3. Slight cross-reactivity to di-methyl K79.
  • Tested Applications & Species

    Application Species
    ChIP
    Human
    IP
    Human
    WB
    Cow
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Histone H3 aa 50 to the C-terminus (mono methyl K79) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    (Peptide available as ab4555)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Methylated
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies

Associated products

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Control Peptide

    • Human Histone H3 (mono methyl K9) peptide (ab1771)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Histone H3 protein (Active) (ab198757)
  • Related Products

    • Histone H3 (mono-methyl K79) Quantification Kit (Colorimetric) (ab115083)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ChIP
Human
IP
Human
WB
Cow
Human
Application Abreviews Notes
ChIP (3)
Use 2µg for 106 cells.
IP
Use at an assay dependent concentration.
WB (5)
1/1 - 1/500. Detects a band of approximately 17 kDa.
PepArr
Use a concentration of 0.2 - 0.02 µg/ml.
Notes
ChIP
Use 2µg for 106 cells.
IP
Use at an assay dependent concentration.
WB
1/1 - 1/500. Detects a band of approximately 17 kDa.
PepArr
Use a concentration of 0.2 - 0.02 µg/ml.

Target

  • Function

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities

    Belongs to the histone H3 family.
  • Developmental stage

    Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
  • Post-translational
    modifications

    Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
    Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
    Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
    Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
    Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
    Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
  • Cellular localization

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P68431 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8350 Human
    • Entrez Gene: 8351 Human
    • Entrez Gene: 8352 Human
    • Entrez Gene: 8353 Human
    • Entrez Gene: 8354 Human
    • Entrez Gene: 8355 Human
    • Entrez Gene: 8356 Human
    • Entrez Gene: 8357 Human
    • Entrez Gene: 8358 Human
    • Entrez Gene: 8968 Human
    • Entrez Gene: 319152 Mouse
    • Entrez Gene: 319153 Mouse
    • Entrez Gene: 360198 Mouse
    • Entrez Gene: 97908 Mouse
    • Entrez Gene: 100364501 Rat
    • Entrez Gene: 100365669 Rat
    • Entrez Gene: 291159 Rat
    • Entrez Gene: 679994 Rat
    • Entrez Gene: 680511 Rat
    • Entrez Gene: 682330 Rat
    • Omim: 602810 Human
    • SwissProt: P68432 Cow
    • SwissProt: P68431 Human
    • SwissProt: P68433 Mouse
    • SwissProt: Q6LED0 Rat
    • Unigene: 132854 Human
    • Unigene: 247813 Human
    • Unigene: 247814 Human
    • Unigene: 248176 Human
    • Unigene: 443021 Human
    • Unigene: 484990 Human
    • Unigene: 532144 Human
    • Unigene: 533292 Human
    • Unigene: 546315 Human
    • Unigene: 586261 Human
    • Unigene: 591778 Human
    • Unigene: 221301 Mouse
    • Unigene: 261657 Mouse
    • Unigene: 377874 Mouse
    • Unigene: 390558 Mouse
    • Unigene: 397328 Mouse
    • Unigene: 138090 Rat
    see all
  • Alternative names

    • H3 histone family member E pseudogene antibody
    • H3 histone family, member A antibody
    • H3/A antibody
    • H31_HUMAN antibody
    • H3F3 antibody
    • H3FA antibody
    • Hist1h3a antibody
    • HIST1H3B antibody
    • HIST1H3C antibody
    • HIST1H3D antibody
    • HIST1H3E antibody
    • HIST1H3F antibody
    • HIST1H3G antibody
    • HIST1H3H antibody
    • HIST1H3I antibody
    • HIST1H3J antibody
    • HIST3H3 antibody
    • histone 1, H3a antibody
    • Histone cluster 1, H3a antibody
    • Histone H3 3 pseudogene antibody
    • Histone H3.1 antibody
    • Histone H3/a antibody
    • Histone H3/b antibody
    • Histone H3/c antibody
    • Histone H3/d antibody
    • Histone H3/f antibody
    • Histone H3/h antibody
    • Histone H3/i antibody
    • Histone H3/j antibody
    • Histone H3/k antibody
    • Histone H3/l antibody
    see all

Images

  • ChIP - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
    ChIP - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)

    Chromatin was prepared from U-2 OS (Human bone osteosarcoma epithelial cell line) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The  ChIP was performed with 25 µg of chromatin, 2 µg of  ab2886 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR. Primers and probes are located in the first kb of the transcribed region.

  • Western blot - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
    Western blot - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
    Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886) at 1 µg/ml + Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

    Secondary
    Human NFIB / NF1B2 peptide (ab95051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15.4 kDa
    Additional bands at: 17 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes
  • Peptide Array - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
    Peptide Array - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)

    All batches of ab2886 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - mono methyl K79 peptide (ab4555), indicating that this antibody specifically recognises the Histone H3 - mono methyl K79 modification.

    ab4558 - Histone H3 - unmodified

    ab1771 - Histone H3 - di methyl K9

    ab4555 - Histone H3 - mono methyl K79

    ab4556 - Histone H3 - di methyl K79

    ab4557 - Histone H3 - tri methyl K79

    ab4560 - Histone H4 - di methyl K79

  • Western blot - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
    Western blot - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
    All lanes : Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886) at 1/300 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Testis (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15.4 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?


    Exposure time: 4 minutes
  • ChIP - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
    ChIP - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)This image is a courtesy of Anonymous Abreview

    Chromatin was prepared from whole cell lysate of normal rat liver and liver cancer cells. The cross-linking (X-ChiP) technique was used, crosslinking was performed for 15 minutes in formaldehyde. 5 µg of the primary antibody was used in 1/100 dilution and it was incubated with the sample for 16 hours at 4°C in a commercially available ChIP dilution buffer. The immunoprecipitated DNA was quantified by real time PCR. ChIP results show that the Histone H3 (mono methyl K79) and GAPDH genes are expressed in higher levels in liver cancer cells than in normal liver cells.

    See Abreview

  • Immunoprecipitation - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
    Immunoprecipitation - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)

    Histone H3 (mono methyl K79) was immunoprecipitated using 0.5mg HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate, 5µg of Rabbit polyclonal to Histone H3 (mono methyl K79) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab2886.
    Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
    Band: 18kDa: Histone H3 (mono methyl K79).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (47)

    Publishing research using ab2886? Please let us know so that we can cite the reference in this datasheet.

    ab2886 has been referenced in 47 publications.

    • Liu C  et al. CBP mediated DOT1L acetylation confers DOT1L stability and promotes cancer metastasis. Theranostics 10:1758-1776 (2020). PubMed: 32042335
    • Nassa G  et al. Inhibition of histone methyltransferase DOT1L silences ERa gene and blocks proliferation of antiestrogen-resistant breast cancer cells. Sci Adv 5:eaav5590 (2019). PubMed: 30775443
    • Chory EJ  et al. Nucleosome Turnover Regulates Histone Methylation Patterns over the Genome. Mol Cell 73:61-72.e3 (2019). PubMed: 30472189
    • Lam KG  et al. Cell-type-specific genomics reveals histone modification dynamics in mammalian meiosis. Nat Commun 10:3821 (2019). PubMed: 31444359
    • Lu X  et al. GLP-catalyzed H4K16me1 promotes 53BP1 recruitment to permit DNA damage repair and cell survival. Nucleic Acids Res 47:10977-10993 (2019). PubMed: 31612207
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 12 Abreviews or Q&A

    Western blot abreview for Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Cell lysate - nuclear (wildtype, histone H3 mutant, dot1 mutant)
    Gel Running Conditions
    Reduced Denaturing (BioRad AnyKD)
    Loading amount
    1e+006 cells
    Treatment
    UV exposed (200 J/m2)
    Specification
    wildtype, histone H3 mutant, dot1 mutant
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Jeff Thompson

    Verified customer

    Submitted Jul 23 2015

    Western blot abreview for Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    4.5 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Fruit fly (Drosophila melanogaster) Tissue lysate - nuclear (whole fruit fly)
    Specification
    whole fruit fly
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Mr. Alexander Hambly

    Verified customer

    Submitted Mar 24 2015

    ChIP abreview for Anti-Histone H3 (methylated) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (Hela)
    Specification
    Hela
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 15 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% Formaldehyde
    Detection step
    Real-time PCR
    Negative control
    No DNA control
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 20 2012

    ChIP abreview for Anti-Histone H3 (methylated) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    Application
    ChIP
    Sample
    Mouse Tissue lysate - nuclear (mouse testis)
    Specification
    mouse testis
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 15 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% Formaldehyde
    Detection step
    Real-time PCR
    Negative control
    No DNA control
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 20 2012

    Western blot abreview for Anti-Histone H3 (methylated) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - nuclear (mouse testis)
    Loading amount
    20 µg
    Specification
    mouse testis
    Gel Running Conditions
    Reduced Denaturing (15%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 20 2012

    Western blot abreview for Anti-Histone H3 (methylated) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (Hela)
    Loading amount
    25 µg
    Specification
    Hela
    Gel Running Conditions
    Reduced Denaturing (15%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 20 2012

    ChIP abreview for Anti-Histone H3 (methylated) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Rat Cell lysate - whole cell (Normal Liver and liver cancer cells)
    Specification
    Normal Liver and liver cancer cells
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 15 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde
    Detection step
    Real-time PCR
    Positive control
    GAPDH
    Negative control
    Normal IgG
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 17 2009

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (methylated) antibody - ChIP Grade

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Sep 12 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (methylated) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (10T1/2)
    Specification
    10T1/2
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Sep 12 2008

    Question

    Our lab bought several antibodies from your company before. Now I am trying to do ChIP and particularly interested in trying the PCR primers you guys used. I notice that the website of your company does not list this information whereas the delivered information sheets do. I am wondering whether you can help me with the sequences of PCR primers for the following antibodies: ab8895, ab8580, ab2886, ab3594, and ab2621. I appreciate if you can mail me information sheets regarding these A/bs.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 20 2007

    Answer

    All antibodies that are batch tested here at Abcam have the primers that were employed during the testing published as a separate page which can be accessed as a clickable link on the antibody datasheet. For example on the antibody datasheet for ab8895 under "specific protocols" there is a clickable link; "PCR primers used to test ab8895 in ChIP at Abcam" Here you will find all the information necessary to test the antibody. You can locate the same PCR primer information on each of the antibody datasheets you refer to.

    Read More

    Abcam Scientific Support

    Answered on Feb 21 2007

    1-10 of 12 Abreviews or Q&A

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