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Epigenetics and Nuclear Signaling Histones H3 Methylated
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Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)

  • Datasheet
  • SDS
Reviews (9) Submit a question References (41)

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ChIP - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)
  • Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)
  • Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)
  • Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)
  • Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)

Key features and details

  • Rabbit polyclonal to Histone H3 (mono methyl K9) - ChIP Grade
  • Suitable for: WB, ChIP, ICC/IF
  • Reacts with: Cow, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant human Histone H3 protein (Active) (ab198757)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
Product image
Anti-Histone H3 (di methyl K9) antibody [Y49] - ChIP Grade (ab32521)

View more associated products

Overview

  • Product name

    Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade
    See all Histone H3 primary antibodies
  • Description

    Rabbit polyclonal to Histone H3 (mono methyl K9) - ChIP Grade
  • Host species

    Rabbit
  • Specificity

    Specific for mono-methyl lysine 9 of Histone H3. There is no cross-reactivity with lysine 27 of Histone H3.
  • Tested Applications & Species

    Application Species
    ChIP
    Human
    ICC/IF
    Human
    WB
    Cow
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Histone H3 aa 1-100 (mono methyl K9).
    (Peptide available as ab1771)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Methylated
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies
    • Signal Transduction
    • Antibodies
    • histone h3

Associated products

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Corresponding Unmodified Peptide

    • Human Histone H3 (unmodified) peptide (ab7228)
  • Immunizing Peptide (Blocking)

    • Human Histone H3 (mono methyl K9) peptide (ab1771)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Histone H3 protein (Active) (ab198757)
  • Related Products

    • Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit (ab113453)
    • Histone H3 (mono-methyl K9) Quantification Kit (Colorimetric) (ab115060)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab8896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ChIP
Human
ICC/IF
Human
WB
Cow
All applications
Caenorhabditis elegans
a wide range of other species
Mammals
Application Abreviews Notes
WB (5)
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).Can be blocked with Human Histone H3 (mono methyl K9) peptide (ab1771).
ChIP (2)
Use 2-25 µg for µg of chromatin.
ICC/IF (2)
Use at an assay dependent concentration.
Notes
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).Can be blocked with Human Histone H3 (mono methyl K9) peptide (ab1771).
ChIP
Use 2-25 µg for µg of chromatin.
ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities

    Belongs to the histone H3 family.
  • Developmental stage

    Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
  • Post-translational
    modifications

    Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
    Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
    Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
    Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
    Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
    Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
  • Cellular localization

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P68431 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8350 Human
    • Entrez Gene: 8351 Human
    • Entrez Gene: 8352 Human
    • Entrez Gene: 8353 Human
    • Entrez Gene: 8354 Human
    • Entrez Gene: 8355 Human
    • Entrez Gene: 8356 Human
    • Entrez Gene: 8357 Human
    • Entrez Gene: 8358 Human
    • Entrez Gene: 8968 Human
    • Omim: 602810 Human
    • SwissProt: P68432 Cow
    • SwissProt: P68431 Human
    • Unigene: 132854 Human
    • Unigene: 247813 Human
    • Unigene: 247814 Human
    • Unigene: 248176 Human
    • Unigene: 443021 Human
    • Unigene: 484990 Human
    • Unigene: 532144 Human
    • Unigene: 533292 Human
    • Unigene: 546315 Human
    • Unigene: 586261 Human
    • Unigene: 591778 Human
    see all
  • Alternative names

    • H3 histone family member E pseudogene antibody
    • H3 histone family, member A antibody
    • H3/A antibody
    • H31_HUMAN antibody
    • H3F3 antibody
    • H3FA antibody
    • Hist1h3a antibody
    • HIST1H3B antibody
    • HIST1H3C antibody
    • HIST1H3D antibody
    • HIST1H3E antibody
    • HIST1H3F antibody
    • HIST1H3G antibody
    • HIST1H3H antibody
    • HIST1H3I antibody
    • HIST1H3J antibody
    • HIST3H3 antibody
    • histone 1, H3a antibody
    • Histone cluster 1, H3a antibody
    • Histone H3 3 pseudogene antibody
    • Histone H3.1 antibody
    • Histone H3/a antibody
    • Histone H3/b antibody
    • Histone H3/c antibody
    • Histone H3/d antibody
    • Histone H3/f antibody
    • Histone H3/h antibody
    • Histone H3/i antibody
    • Histone H3/j antibody
    • Histone H3/k antibody
    • Histone H3/l antibody
    see all

Images

  • ChIP - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)
    ChIP - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)

    Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 2µg of  ab8896 (blue), and 20µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

  • Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)
    Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)This image is courtesy of an anonymous Abreview
    Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896) at 1/300 dilution (Incubated for 10 minutes at 20°C. Diluent buffer used was 0.5% milk in TBS, 0.1% Tween.) + HUH-7 cells at 15 µg

    Secondary
    Sheep anti-rabbit IgG (HRP) at 1/20000 dilution

    Predicted band size: 15 kDa

    See Abreview

  • Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)
    Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)
    All lanes : Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896) at 1 µg/ml

    Lane 1 : Calf Thymus Histone Preparation
    Lane 2 : Calf Thymus Histone Preparation with Human Histone H3 (mono methyl K9) peptide (ab1771) at 0.5 µg/ml
    Lane 3 : Calf Thymus Histone Preparation with Human Histone H3 (di methyl K9) peptide (ab1772) at 0.5 µg/ml
    Lane 4 : Calf Thymus Histone Preparation with Human Histone H3 (tri methyl K9) peptide (ab1773) at 0.5 µg/ml
    Lane 5 : Calf Thymus Histone Preparation with Human Histone H3 (mono methyl K27) peptide (ab1780) at 0.5 µg/ml
    Lane 6 : Calf Thymus Histone Preparation with Human Histone H3 (di methyl K27) peptide (ab1781) at 0.5 µg/ml
    Lane 7 : Calf Thymus Histone Preparation with Human Histone H3 (tri methyl K27) peptide (ab1782) at 0.5 µg/ml
    Lane 8 : Calf Thymus Histone Preparation with Human Histone H3 (mono methyl K4) peptide (ab1340) at 0.5 µg/ml
    Lane 9 : Calf Thymus Histone Preparation with Human Histone H3 (unmodified) peptide (ab7228) at 0.5 µg/ml
    Lane 10 : Calf Thymus Histone Preparation with Human Histone H3 (unmodified ) peptide (ab2623) at 0.5 µg/ml

    Lysates/proteins at 0.5 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?



    ab8896 specifically recognises mono-methyl K9 (lane 1) in calf thymus lysate at 17kDa. ab8896 is successfully blocked using the immunizing peptide (lane 2 ab1771), but not the di-methyl K9 (lane 3 ab1772), the tri-methyl K9 (lane 4 ab1773), the mono-methyl K27 (lane 5 ab1780), the di-methyl K27 (lane 6 ab1781), the tri-methyl K27 (lane 7 ab1782), the mono-methyl K4 (lane 8 ab1340) nor the corresponding unmodified peptides (lane 9 ab7228, lane 10 ab2623). This implies that ab8896 is specific to mono-methyl K9.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)This image is courtesy of an anonymous Abreview

    Ab8896 staining Histone H3 (mono methyl K9) in HeLa (Cervical cancer cell line) cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton/TBS and blocked with 2% serum for 30 minutes at 25°C. Samples were incubated with primary antibody at 1/100 dilution for 1 hour at 25°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit was used as a secondary antibody at a 1/1000 dilution.

    See Abreview

  • Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)
    Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)This image is courtesy of an anonymous Abreview
    Diluent: TBS + 0.1% Tween 20 + 5% BSA. Incubated for 12 hours at 4°C + HeLa whole cell lysates at 20 µg

    Secondary
    Anti-rabbit IgG, HRP-linked Antibody at 1/2000 dilution

    Predicted band size: 15 kDa


    Exposure time: 1 minute

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)This image is courtesy of an Abreview submitted by Kirk Mcmanus.

    Ab8896 staining Histone H3 (mono methyl K9) in HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.05% Triton X100 in PBS. Samples were incubated with primary antibody at 1/200 dilution in PBS for 1 hour at 22°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit was used as the secondary antibody at a 1/200 dilution.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)

    ICC/IF image of ab8896 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab8896 at 1µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was a goat anti-rabbit Alexa Fluor® 488 (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)
    Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)This image is courtesy of an anonymous abreview.
    All lanes : Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896) at 1/1000 dilution

    Lane 1 : Nuclear lysates prepared from Hela cell: SiRNA untreated
    Lane 2 : Nuclear lysates prepared from Hela cells treated with G9a SiRNA

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Alkaline Phosphatase conjugated rabbit polyclonal rabbit IgG (Fc) at 1/5000 dilution

    Predicted band size: 15 kDa
    Observed band size: 16 kDa why is the actual band size different from the predicted?


    Exposure time: 10 minutes

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (41)

    Publishing research using ab8896? Please let us know so that we can cite the reference in this datasheet.

    ab8896 has been referenced in 41 publications.

    • Jang JE  et al. PERK/NRF2 and autophagy form a resistance mechanism against G9a inhibition in leukemia stem cells. J Exp Clin Cancer Res 39:66 (2020). PubMed: 32293500
    • Orouji E  et al. Unique Role of Histone Methyltransferase PRDM8 in the Tumorigenesis of Virus-Negative Merkel Cell Carcinoma. Cancers (Basel) 12:N/A (2020). PubMed: 32344701
    • Clarke TL  et al. Histone Lysine Methylation Dynamics Control EGFR DNA Copy-Number Amplification. Cancer Discov 10:306-325 (2020). PubMed: 31776131
    • Roy A  et al. IFI16, a nuclear innate immune DNA sensor, mediates epigenetic silencing of herpesvirus genomes by its association with H3K9 methyltransferases SUV39H1 and GLP. Elife 8:N/A (2019). PubMed: 31682228
    • Akoury E  et al. Disordered region of H3K9 methyltransferase Clr4 binds the nucleosome and contributes to its activity. Nucleic Acids Res 47:6726-6736 (2019). PubMed: 31165882
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-9 of 9 Abreviews or Q&A

    Western blot abreview for Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (B Cells)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    20 µg
    Specification
    B Cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 05 2018

    Western blot abreview for Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Caenorhabditis elegans Tissue lysate - nuclear (C. elegans whole body)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    4.5 µg
    Specification
    C. elegans whole body
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 28 2013

    Western blot abreview for Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (12)
    Sample
    Human Cell lysate - whole cell (Human HeLa cells)
    Specification
    Human HeLa cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 28 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (methylated) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.05% Triton X100 in PBS
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Feb 19 2013

    Western blot abreview for Anti-Histone H3 (methylated) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (huh-7 cells)
    Loading amount
    15 µg
    Specification
    huh-7 cells
    Treatment
    cells expressing recombitant protein
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 04 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (methylated) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa (cervical cancer cell line))
    Specification
    HeLa (cervical cancer cell line)
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.3% Triton/TBS
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 15 2010

    ChIP abreview for Anti-Histone H3 (methylated) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (K562)
    Specification
    K562
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% formaldehyde
    Detection step
    Semiquantitative PCR
    Positive control
    USP3
    Negative control
    HNRPA2B1 was used as a negative control as seen using publicly available ChIP-Seq data in K562 cells.
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 01 2010

    ChIP abreview for Anti-Histone H3 (methylated) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (HeLa)
    Specification
    HeLa
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% formaldehyde
    Detection step
    Real-time PCR
    Positive control
    bglobin
    Negative control
    GAPDH
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    Abcam user community

    Verified customer

    Submitted Mar 18 2010

    Western blot abreview for Anti-Histone H3 (methylated) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (HeLa)
    Loading amount
    10 µg
    Specification
    HeLa
    Treatment
    G9a specific siRNA
    Gel Running Conditions
    Reduced Denaturing (15% gel)
    Blocking step
    Milk as blocking agent for 15 minute(s) · Concentration: 2% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 23 2009

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