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Methylation of histone H3 at lysine 9 by ESET, G9a among other methyl transferases mediates heterochromatin formation by forming a binding site for HP1. H3 (mono-methyl K9) is enriched in certain euchromatin domains which have been postulated to be transcriptionally silent.
Histone H3 (mono-methyl K9) Quantification Kit (Fluorometric) (ab115061) allows the user to specifically measure global mono-methylation of histone H3K9 fluorometrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.
|Components||48 tests||96 tests|
|10X Wash Buffer||1 x 10ml||1 x 20ml|
|8-Well Sample Strips (with Frame)||4 units||9 units|
|8-Well Standard Control Strips||2 units||3 units|
|Antibody Buffer||1 x 6ml||1 x 12ml|
|Detection Antibody, 1 mg/mL||1 x 5µl||1 x 10µl|
|Fluoro Developer||1 x 12µl||1 x 24µl|
|Fluoro Dilution||1 x 4ml||1 x 8ml|
|Fluoro Enhancer||1 x 12µl||1 x 24µl|
|Standard Control, 100 µg/mL||1 x 10µl||1 x 20µl|
Histone extracts were prepared from MDA-231 cells using the Histone Extraction Kit (ab113476) and the amount of mono-methyl H3K9 was measured using ab115061.
ab115061 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"