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Histone H3 Peptide Array Kit (96-well plate-based) (ab233494)

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  • Protocol Booklet
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Graphical analysis of specificity and binding intensity of H3K9me1 antibody.

    Key features and details

    • Detection method: Colorimetric
    • Platform: Microplate

    Overview

    • Product name

      Histone H3 Peptide Array Kit (96-well plate-based)
    • Detection method

      Colorimetric
    • Assay duration

      Multiple steps standard assay
    • Product overview

      The Histone H3 Peptide Array ELISA Kit (96-well plate-based) (ab233494) is designed to rapidly examine the selectivity and specificity of antibodies against 46 different histone modifications and is also suitable for identifying substrates of histone modifying enzymes and analyzing specificity of histone binding proteins.


      In an assay with this kit, the Histone H3 proteins (modified at specific sites) that are tightly arrayed on the wells are incubated with input materials such as antibodies, proteins or enzymes. After incubation, the input materials are detected through an ELISA reaction system, the binding intensity is proportional to the intensity of the absorbance.


       

    • Notes

      Histone modifications have also been defined as epigenetic modifiers. Post-translational modifications of histones include the acetylation on specific lysine residues by histone acetyltransferases (HATs), the deacetylation by histone deacetylases (HDACs), the methylation of lysine and arginine residues by histone methytransferases (HMTs), the demethylation of lysine residues by histone demethylases (HDMTs), and the phosphorylation of specific serine groups by histone kinases (HKs). Next to DNA methylation, histone acetylation and histone methylation are the most well-characterized epigenetic marks. Generally, tri-methylation at H3-K4, H3-K36, or H3-K79 results in an open chromatin configuration and is, therefore, characteristic of euchromatin.

       

       

    • Tested applications

      Suitable for: PepArrmore details
    • Platform

      Microplate

    Properties

    • Storage instructions

      Please refer to protocols.
    • Components 1 x 96 tests
      10X Wash Buffer 1 x 28ml
      Adhesive Covering Film 1 unit
      Array Plate (96-well) 1 unit
      Developer Solution 1 x 12ml
      Stop Solution 1 x 10ml

    Applications

    Our Abpromise guarantee covers the use of ab233494 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    PepArr Use at an assay dependent concentration.

    Images

    • Graphical analysis of specificity and binding intensity of H3K9me1 antibody.
      Graphical analysis of specificity and binding intensity of H3K9me1 antibody.

      The Histone H3 Array was probed with a H3K9me1 polyclonal antibody. Peptides and control were visualized using a goat anti-rabbit IgG-HRP secondary antibody.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab233494? Please let us know so that we can cite the reference in this datasheet.

    ab233494 has not yet been referenced specifically in any publications.

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