Key features and details
- Detection method: Colorimetric
- Platform: Microplate
Product nameHistone H3 Peptide Array Kit (96-well plate-based)
Assay durationMultiple steps standard assay
The Histone H3 Peptide Array ELISA Kit (96-well plate-based) (ab233494) is designed to rapidly examine the selectivity and specificity of antibodies against 46 different histone modifications and is also suitable for identifying substrates of histone modifying enzymes and analyzing specificity of histone binding proteins.
In an assay with this kit, the Histone H3 proteins (modified at specific sites) that are tightly arrayed on the wells are incubated with input materials such as antibodies, proteins or enzymes. After incubation, the input materials are detected through an ELISA reaction system, the binding intensity is proportional to the intensity of the absorbance.
Histone modifications have also been defined as epigenetic modifiers. Post-translational modifications of histones include the acetylation on specific lysine residues by histone acetyltransferases (HATs), the deacetylation by histone deacetylases (HDACs), the methylation of lysine and arginine residues by histone methytransferases (HMTs), the demethylation of lysine residues by histone demethylases (HDMTs), and the phosphorylation of specific serine groups by histone kinases (HKs). Next to DNA methylation, histone acetylation and histone methylation are the most well-characterized epigenetic marks. Generally, tri-methylation at H3-K4, H3-K36, or H3-K79 results in an open chromatin configuration and is, therefore, characteristic of euchromatin.
Tested applicationsSuitable for: PepArrmore details
Storage instructionsPlease refer to protocols.
Components 1 x 96 tests 10X Wash Buffer 1 x 28ml Adhesive Covering Film 1 unit Array Plate (96-well) 1 unit Developer Solution 1 x 12ml Stop Solution 1 x 10ml
Our Abpromise guarantee covers the use of ab233494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|PepArr||Use at an assay dependent concentration.|
ab233494 has not yet been referenced specifically in any publications.