• Product name

    Histone H3 Total Acetylation Detection Fast Kit (Colorimetric)
    See all Histone H3 acetylation kits
  • Detection method

  • Sample type

    Tissue, Adherent cells, Suspension cells
  • Sensitivity

    2 ng
  • Range

    2 ng - 100 ng
  • Assay time

    2h 30m
  • Species reactivity

    Reacts with: Mouse, Cow, Human
    Predicted to work with: Mammals
  • Product overview

    Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 actylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. The reversible lysine acetylation of histone H3 may play a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication and repair, nuclear import and neuronal repression.
    Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) allows the user to colorimetrically detect and quantify if histone H3 is acetylated. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. ab115124 is suitable for specifically measuring total histone H3 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.

  • Platform

    Microplate reader



  • The differences in H3 acetylation level in fetal livers (female fetuses aged 19 dpc) from F1, F2, and F3 generations. measured with ab113476. Histones were prepared using ab115124
    Asterisk indicate a significant difference between the respective C and R groups: ∗∗ P < 0.01; NS not significant. Data presented as the means ± SE (n = 6 per group).

  • Acetylation Histone 3 per mg of extracted histones from murine tissue preparations (extracted using ab113476; 20-500 ng per well) (duplicates +/- SD).

  • Standard curve with background signal subtracted (duplicates; +/- SD).



This product has been referenced in:

  • Nowacka-Woszuk J  et al. Transgenerational effects of prenatal restricted diet on gene expression and histone modifications in the rat. PLoS One 13:e0193464 (2018). Read more (PubMed: 29474484) »
  • Zhao B  et al. The BET-bromodomain inhibitor JQ1 mitigates vemurafenib drug resistance in melanoma. Melanoma Res 28:521-526 (2018). Read more (PubMed: 30192303) »
See all 5 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

This assay was performed on histones isolated from the flesh fly, Sarcophaga bullata. Histones were isolated from diapause and control pupae using the Histone Extraction kit (ab113476) and had been stored at -80 °C for approximately 6 months before being used in the assay. The total protein for each sample was estimated by measuring absorbance at 280 nm with a nanodrop spectrophotometer. All samples were diluted to 1 µg of protein using the Antibody buffer provided with the kit. A range of protein amounts (1-10 µg) were used in the assay.
The assay was conducted according to the provided instructions. Samples and standards were incubated at 25 °C for ~ 100 minutes before proceeding with the first wash step. Average, blank corrected, O.D. values for acetylated standards ranged from 0.221 to 5.86 the curve was approximately logarithmic (see below). The average, blank corrected, O.D. values ranged from 0.014 to 0.1 for control samples and 0.006 to 0.283 for diapause samples. Only the 10 µg per well diapause samples had O.D. values within the range of the standards. The control samples appeared to have ~ 50 % less acetylated histone protein than the diapause samples (see figure below), as was expected from our previous research.
In summary, the kit was easy to use and does appear to work with our non-model insect.

Dr. Julie Reynolds

Verified customer

Submitted Feb 08 2016


The detection antibody in ab115124 kit is labeled with signal reporter already. But H4 antibody included in the kit ab115125 is not and needs to be labeled by signal reporter and enhanced with enhancer during the assay.

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1. Yes, it is possible for tissue or cell pellets to be frozen before extracting histone proteins.

2. The minimum amount of histone protein required as input for these assays are 50 ng (both for the colorimetric and fluorometric kits). In principle, 50 ng of histone proteins could be obtained from as little as 100,000 cells so pooling of blood may not be necessary. However we recommend using 1 million cells if possible in order to obtain a suitable amount of histone proteins for the assay.

3. We recommend using any standard protein quantification assay such as a Bradford assay for histone quantification.

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Compared to ab115102, ab115124 is much faster and easier to handle with higher sensitivity and better reproducibility, but slightly less specificity. 1-2 ug of histone protein (min 50,000 cells) may be needed for each assay point if using ab115102, while only 50-100 ng of histone protein (min 5000 cells) are required for each point with use of ab115124. For both kits, the CV% is less than 8% between the wells and less than 12% between the strips.

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Thank you for contacting us. We have not tested these kits in fish and have no testing data on this use. The kit ab115103 may be suitable for use in fish based on principle. However, the kit ab115124 may not be suitable for fish.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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The kits are very similar. The main differences are that the ab115102 requires 5 hrs to run and you need to coat the antibody yourself while the kit ab115124 only requires 2.5 hours and the wells come pre-coated.

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