Key features and details
- Rabbit polyclonal to Histone H3 (tri methyl K56)
- Suitable for: WB, Dot blot
- Reacts with: Human, Caenorhabditis elegans
- Isotype: IgG
Product nameAnti-Histone H3 (tri methyl K56) antibody
See all Histone H3 primary antibodies
DescriptionRabbit polyclonal to Histone H3 (tri methyl K56)
Tested applicationsSuitable for: WB, Dot blotmore details
Species reactivityReacts with: Human, Caenorhabditis elegans
Synthetic peptide corresponding to Human Histone H3 (tri methyl K56).
Database link: P68431
- WB: Caenorhabditis elegans embryo lysate. ICC: HeLa cells (prophase and telophase).
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.0004% Potassium phosphate, 0.0008% Sodium chloride, 30% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272162 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 15 kDa.
1/1000. Predicted molecular weight: 15 kDa.
FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similaritiesBelongs to the histone H3 family.
Developmental stageExpressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
modificationsAcetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
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Immunofluorescence analysis of ab272162. HeLa (Human epithelial cell line from cervix adenocarcinoma) cells are shown during prophase. Primary antibody ab272162 was used at 1:100 dilution for 1 hour at room temperature. Secondary antibody, Dylight 488 antibody at 1:10,000 for 45 minutes at room temperature. Histone H3 [Trimethyl Lys56] localization is nuclear and chromosomal and it is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red). Fixation was 0.5% PFA. Antigen retrieval was not required.
Anti-Histone H3 (tri methyl K56) antibody (ab272162) at 1 µg/ml + Caenorhabditis elegans embryo lysate at 30 µg
IRDye800™ rabbit secondary antibody at 1/10000 dilution
Predicted band size: 15 kDa
Block: 5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~15 kDa.
Other band(s): None.
Dot Blot analysis of ab272162.
Lane 1: Ac.
Lane 2: me1.
Lane 3: me2.
Lane 4: me3.
Lane 5: unmodified.
Load: 1, 10, and 100 picomoles of peptide. Primary antibody ab272162 was used at 1:40 for 45 minutes at 4°C. Secondary antibody Dylight™488 rabbit antibody at 1:10,000 for 45 minutes at room temperature. Block: 5% BLOTTO overnight at 4°C.
Immunofluorescence of ab272162. HeLa (Human epithelial cell line from cervix adenocarcinoma) cells are shown during telophase. Primary antibody ab272162 was at a 1:100 dilution for 1 hour at room temperature. Dylight 488 secondary antibody followed at 1:10,000 for 45 minutes at toom temperature. Histone H3 [Trimethyl Lys56] localization is nuclear and chromosomal. Histone H3 [Trimethyl Lys56] staining is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red). Fixation was 0.5% PFA and antigen retrieval was not required.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272162 has not yet been referenced specifically in any publications.