Application
ChIP
Sample
Mouse Cell lysate - other (mouse ES cells, 300,000 cells, digitonin 0.03% in)
Specification
mouse ES cells, 300,000 cells, digitonin 0.03% in
Detection step
Other
Type
Native ChIP (N-ChIP)
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200
Additional data
Additional Notes
This is actually not ChIP. I performed CUT&RUN and want to write a review about it but there's no such option in the drop-down menu. The detection was done by running the precipitated DNA on a bioanalyser with high sensitivity DNA assay. The result is shown in the picture. The presence of peak at ~150 and 300bp and a high molecular weight band indicated specific recognition of H3K9me3 and release of DNA by the pA-MN cutting.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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Submitted Feb 03 2020