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Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade

Excellent
Abreviews
Go to datasheet (ab8898)
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Pancreatic tumor)
Permeabilization
Yes - 0.1% TritonX100
Specification
Pancreatic tumor
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde

Other product details

Incubation time
3 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 5% BSA
Dilution
1/500

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: anti Rb Alexa488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
Used in the following publication: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7259520/#sd
PubMed ID
32434991: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Submitted Apr 21 2021