Lane 1 - 501 mel (human derived melanoma cell line). Lane 2 - SKmel147 (human derived melanoma cell line). 15 kDa is H3, as would be expected in both lanes. There are multiple distinct nonspecific bands in both lanes. I also tried 1:10,000 dilution, but saw no difference in background.
Thank you for sharing this data with us. ab176916 has previously been tested in western blot on HeLa and NIH3T3 whole cell lysates (please see our western blot image on the datasheet). However, based on the data shown here, it seems that not all protocols are equally suitable for ab176916, in western blotting. We would recommend trying this antibody at a much highly dilution >1:20,000 under your conditions. We welcome feedback from other researchers currently using this antibody.
Abcam user community
Submitted Dec 23 2016
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