Western blot abreview for Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade

Good
Abreviews
Application
Western blot
Sample
Human Purified protein (K-562)
Gel Running Conditions
Reduced Denaturing (18% gel)
Loading amount
0.5 µg
Specification
K-562
Blocking step
Odyssey Blocking Buffer (TBS) (LI-COR) 1:1 mixture of Licor blocking buffer and 1X PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey Blocking Buffer (TBS) (LI-COR)
Dilution
1/1000

Secondary antibody

Dilution
1/20000
Name
Non-Abcam antibody was used: Donkey-anti-rabbit-IRDYE 800
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
Odyssey Imaging system (CLX)
Negative control
Recombinant octamer
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 17 kDa
Positive control
Native octamer

Additional data

Additional Notes
H3K9me3 gives a strong band on native H3 and none in recombinant. Weak background on Native H4 and H1. N is native octamer from K562 cells. R is recombinant human octamer purified from bacteria. Revert protein stain is from LI-COR (REVERT™ Total Protein Stain).

Dr. Ragnhild Eskeland

Verified customer

Submitted Oct 27 2016

Sign up