Western blot abreview for Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade

Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cells and HEK293 cells)
Gel Running Conditions
Reduced Denaturing (18)
Loading amount
200000 cells
Treatment
HEK293 cells transfected with SETDB1
Specification
mouse embryonic stem cells and HEK293 cells
Blocking step
(agent) for 12 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 4°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: Odyssey Blocking Buffer (TBS) 50%
Dilution
1/1000

Secondary antibody

Dilution
1/20000
Name
Non-Abcam antibody was used: Donkey-anti-rabbit-IRDYE 800
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye­ 800CW

Detection

Detection method
Odyssey Imaging system (CLX)
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 15 kDa
Positive control
Probed with goat polyclonal anti-H3 ab12079.

Additional data

Additional Notes
1 HEK293 2x10^5 2 HEK293 2x10^5 transfected with human SETDB1 3 mouse Embryonic stem cells 2x10^5 Observe a few weak background bands at 25-100 kDa, however these bands are also detected with the H3 antibody ab12079.

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Submitted Dec 30 2016

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