Flow Cytometry abreview for Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade

Excellent
Abreviews
Application
Flow Cytometry
Sample
Mouse Cell (T lymphocytes)
Permeabilization
Yes - eBioscience FoxP3/TF staining kit
Gating Strategy
Gated on CD3+/Thy+ cells
Specification
T lymphocytes
Preparation
Cell harvesting/tissue preparation method: Extracted from spleen, by magnetic negative selection
Sample buffer: FACS buffer (PBS + 2% SVF + 0.5 mM EDTA + 0.01% azide)
Fixation
Formaldehyde

Other product details

Incubation time
15 minute(s) · Temperature: 4°C
Dilution
1/100

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Goatanti-rabbit IgG-AF488 (Molecular Probes # A110
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 488

Abcam user community

Verified customer

Submitted Jun 20 2017

Sign up