Anti-Histone H3 (tri methyl K9, phospho S10) antibody - ChIP Grade (ab5819)


  • Product name
    Anti-Histone H3 (tri methyl K9, phospho S10) antibody - ChIP Grade
    See all Histone H3 primary antibodies
  • Description
    Rabbit polyclonal to Histone H3 (tri methyl K9, phospho S10) - ChIP Grade
  • Host species
  • Tested applications
    Suitable for: IHC-P, ChIP, WB, ICC/IF, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Histone H3 aa 1-100 (methyl K9, phospho S10) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab15644)

  • Positive control
    • HeLa Histone Preparation; Colcemid-treated HeLa histone Preparation; Hela whole cell extract



Our Abpromise guarantee covers the use of ab5819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ChIP Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 17 kDa).Can be blocked with Human Histone H3 (tri methyl K9 or phospho S10) peptide (ab15644).
ICC/IF Use at an assay dependent concentration.
ICC 1/2000.



  • Rabbit polyclonal to Histone H3 (phospho S10, tri methyl K9) - ab5819, on histone preparations from HeLa cells. Primary antibody used at 1/500.

    Lanes 1, 3, 5, 7, 9 - control HeLa histones (0.5ug/lane)
    Lanes 2, 4, 6, 8, 10 - Colcemid-treated HeLa histones (0.5ug/lane)

    All blocking peptides are 1ug/lane

    Lanes 1 and 2: ab5819  
    Lanes 3 and 4: ab5819 + Histone H3 (Tri Me K9, phospho S10) peptide - ab15644
    Lanes 5 and 6: ab5819 + Histone H3 peptide (unmodified) - ab7228
    Lanes 7 and 8: ab5819 + Histone H3  (phospho S10) peptide - ab11477
    Lanes 9 and 10: ab5819 + Histone H3 (tri methyl K9) peptide - ab1773 

    Secondary antibody - Goat polyclonal to rabbit IgG (HRP) - ab6721 1/5000.

    ab5819 is specific for Histone H3 phosphorylated at residue S10 and tri methylated at residue K9. The activity of the antibody is blocked by the addition of the immunizing peptide, ab15644 (lanes 3 and 4).

  • SKN cells stained with ab5819 (green) at a dilution of 1/2000. The cells were fixed in paraformaldehyde for 10 minutes prior to incubation with ab5819. The DNA is stained with DAPI (blue). 100x magnification.

  • ab5819 at a 1/500 dilution staining mitotic human HeLa cells by immunocytochemistry. The antibody was incubated with formaldehyde fixed cells for 1 1/2 hours and bound antibody was then detected using a Texas red conjugated goat anti-rabbit IgG.

    This image is courtesy of an Anonymous Abreview submitted on 6 April 2006.

    See Abreview

  • IHC image of Histone H3 (tri methyl K9, phospho S10) staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5819, 5µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • References

    This product has been referenced in:
    • Rothbart SB  et al. An Interactive Database for the Assessment of Histone Antibody Specificity. Mol Cell 59:502-11 (2015). Read more (PubMed: 26212453) »
    • Sabbattini P  et al. An H3K9/S10 methyl-phospho switch modulates Polycomb and Pol II binding at repressed genes during differentiation. Mol Biol Cell 25:904-15 (2014). ChIP ; Mouse . Read more (PubMed: 24430871) »

    See all 9 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Immunocytochemistry/ Immunofluorescence
    Human Cell (HeLa)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%

    Abcam user community

    Verified customer

    Submitted Apr 06 2006


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