Asynchronous HeLa cells, were trypsinized, pelleted and fixed with ice cold ethanol overnight. 2x10^6 cells were immunofluorescently labeled for 1hr at room temp with the primary and then for 30 min with a secondary containing propidium iodide and RNase A at 37'C in the dark. This antibody gives a strong mitotic signal that was also confirmed by looking at the labeled cells under the microscope. This antibody does not give as strong a mitotic signal as the rabbit polyclonal ab5176, however it still clearly distinguishes the mitotic cells - there appears to be more background (i.e. interphase) staining in these cells which was confirmed by digital imaging microscopy.
Dr. Kirk Mcmanus
Submitted Nov 10 2008
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