Non-Abcam antibody was used: alexa 555 donkey anti mouse Host species: Donkey Clonality: Polyclonal Conjugation: Alexa Fluor® 555
H3 is in red and dapi is in blue. I probably could have used less H3 antibody. The staining is beautiful and specific with barely any background. This section is 50um thick. As said in the application, the brain was fixed with 4% pfa and embedded in paraffin for sectioning.
Abcam user community
Submitted Apr 20 2009
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