Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] - ChIP Grade

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (E18 Mouse Brain Sections)
Specification
E18 Mouse Brain Sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 1% Triton in PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 1% Donkey Serum + 0.1% Triton

Secondary antibody

Name
Non-Abcam antibody was used: alexa 555 donkey anti mouse
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555

Additional data

Additional Notes
H3 is in red and dapi is in blue. I probably could have used less H3 antibody. The staining is beautiful and specific with barely any background. This section is 50um thick. As said in the application, the brain was fixed with 4% pfa and embedded in paraffin for sectioning.

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Submitted Apr 20 2009

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