Western blot abreview for Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] - ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - nuclear (H1299)
Loading amount
5 µg
Specification
H1299
Treatment
100 ng/ml PMA for 2 h
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% milk

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
eCL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 17 kDa

Additional data

Additional Notes
WB type of membrane is PVDF(0.22um).
Histones are extract by acid-extraction method

Dr. yipeng du

Verified customer

Submitted Mar 01 2010

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