Figure: IHC and confocal microscopy for Histone H3 (phospho S10) (PH3) in the embryonic rat pineal gland. (A) Negative control (-) by omission of the anti-Histone H3 (phospho S10). (B and C) Immunolabeling for PH3 (red) and Pax6 (green). White arrowheads: mitotic cells positive for both markers and located in the luminal side of the committed neuroepithelium. Pax6: transcription factor essential for pineal gland morphogenesis. Scale bar: 10 ´m. Image generated by Maria P. Ibaðez Rodriguez, IHEM, UNCuyo, CONICET, Mendoza, Argentina.