Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] - ChIP Grade

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Embryonic rat pineal gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate buffer pH 6
Permeabilization
No
Specification
Embryonic rat pineal gland
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS with 2% donkey serum
Dilution
1/100

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Anti-mouse antibody (Jackson 715- 165-151)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ­

Additional data

Additional Notes
Figure: IHC and confocal microscopy for Histone H3 (phospho S10) (PH3) in the embryonic rat pineal gland. (A) Negative control (-) by omission of the anti-Histone H3 (phospho S10). (B and C) Immunolabeling for PH3 (red) and Pax6 (green). White arrowheads: mitotic cells positive for both markers and located in the luminal side of the committed neuroepithelium. Pax6: transcription factor essential for pineal gland morphogenesis. Scale bar: 10 ´m. Image generated by Maria P. Ibaðez Rodriguez, IHEM, UNCuyo, CONICET, Mendoza, Argentina.
PubMed ID
27861587: View

Dr. Estela Munoz

Verified customer

Submitted Mar 08 2017

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