Western blot abreview for Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] - ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Gel Running Conditions
Reduced Denaturing (7% Tris Bicine)
Loading amount
50 µg
Treatment
1 mM hydroxyurea for 24 hrs
Specification
U2OS
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 5% milk in TBST
Dilution
1/5000

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Goat anti-mouse (Dako)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 15 kDa
Positive control
Hydroxyurea (block in S phase reduces the mitotic H3 phospho-S10 mark)

Additional data

Additional Notes
Lane 1 = untreated Lane 2 = 1 mM HU, 24 hrs. Nitrocellulose membrane lysis buffer from Laemmli (Nature, 1970)

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Submitted Sep 28 2017

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