Anti-Histone H4 (2-hydroxyisobutyryl K16) antibody (ab235006)
Key features and details
- Rabbit polyclonal to Histone H4 (2-hydroxyisobutyryl K16)
- Suitable for: WB, ICC/IF, IP, ChIP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H4 (2-hydroxyisobutyryl K16) antibody
See all Histone H4 primary antibodies -
Description
Rabbit polyclonal to Histone H4 (2-hydroxyisobutyryl K16) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IP, ChIPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Histone H4 (2-hydroxyisobutyryl K16).
Database link: P62805 -
Positive control
- WB: HeLa, HEK-293, A549 and HepG2 whole cell lysates, treated with 30 mM sodium butyrate for 4 hours. ICC/IF: HeLa cells (treated with 30mM sodium butyrate for 4 hours). ChIP: Chromatin prepared from HeLa cells (treated with 30mM sodium butyrate for 4 hours). IP: HepG2 whole cell lysate, treated with 30 mM sodium butyrate for 4 hours.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab235006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/5000. Predicted molecular weight: 11 kDa. | |
| ICC/IF | 1/50 - 1/200. | |
| IP | 1/200 - 1/2000. | |
| ChIP | Use at an assay dependent concentration. Use 5µg for 4 x 106 cells. |
Target
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Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
Sequence similarities
Belongs to the histone H4 family. -
Post-translational
modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 121504 Human
- Entrez Gene: 554313 Human
- Entrez Gene: 8294 Human
- Entrez Gene: 8359 Human
- Entrez Gene: 8360 Human
- Entrez Gene: 8361 Human
- Entrez Gene: 8362 Human
- Entrez Gene: 8363 Human
see all -
Alternative names
- dJ160A22.1 antibody
- dJ160A22.2 antibody
- dJ221C16.1 antibody
see all
Images
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Western blot - Anti-Histone H4 (2-hydroxyisobutyryl K16) antibody (ab235006)All lanes : Anti-Histone H4 (2-hydroxyisobutyryl K16) antibody (ab235006) at 1/2000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 5 : HeLa whole cell lysate, untreated
Lane 6 : HEK-293 whole cell lysate, untreated
Lane 7 : A549 whole cell lysate, untreated
Lane 8 : HepG2 whole cell lysate, untreated
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 11 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (2-hydroxyisobutyryl K16) antibody (ab235006)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H4 (2-hydroxyisobutyryl K16) using ab235006 at 1/5 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
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ChIP - Anti-Histone H4 (2-hydroxyisobutyryl K16) antibody (ab235006)HeLa (human epithelial cell line from cervix adenocarcinoma) (4 x 106, treated with 30 mM sodium butyrate for 4 hours) cells were treated with Benzanase, sonicated, and immunoprecipitated with 5 μg ab235006 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
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Immunoprecipitation - Anti-Histone H4 (2-hydroxyisobutyryl K16) antibody (ab235006)Histone H4 (2-hydroxyisobutyryl K16) was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate (treated with 30 mM sodium butyrate for 4 hours) using ab235006 at 1/200 dilution.
Lane 1: Rabbit control IgG in HepG2 whole cell lysate (treated with 30 mM sodium butyrate for 4 hours).
Lane 2: ab235006 IP in HepG2 whole cell lysate (treated with 30 mM sodium butyrate for 4 hours).
Lane 3: HepG2 whole cell lysate (treated with 30 mM sodium butyrate for 4 hours) (20 µg input).
For western blotting an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab235006 has not yet been referenced specifically in any publications.
