Anti-Histone H4 (2-hydroxyisobutyryl K16) antibody (ab235006)
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- References
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Overview
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Product name
Anti-Histone H4 (2-hydroxyisobutyryl K16) antibody
See all Histone H4 primary antibodies -
Description
Rabbit polyclonal to Histone H4 (2-hydroxyisobutyryl K16) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IP, ChIPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Histone H4 (2-hydroxyisobutyryl K16).
Database link: P62805 -
Positive control
- WB: HeLa, HEK-293, A549 and HepG2 whole cell lysates, treated with 30 mM sodium butyrate for 4 hours. ICC/IF: HeLa cells (treated with 30mM sodium butyrate for 4 hours). ChIP: Chromatin prepared from HeLa cells (treated with 30mM sodium butyrate for 4 hours). IP: HepG2 whole cell lysate, treated with 30 mM sodium butyrate for 4 hours.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab235006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/5000. Predicted molecular weight: 11 kDa. | |
| ICC/IF | 1/50 - 1/200. | |
| IP | 1/200 - 1/2000. | |
| ChIP | Use at an assay dependent concentration. Use 5µg for 4 x 106 cells. |
Target
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Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
Sequence similarities
Belongs to the histone H4 family. -
Post-translational
modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 121504 Human
- Entrez Gene: 554313 Human
- Entrez Gene: 8294 Human
- Entrez Gene: 8359 Human
- Entrez Gene: 8360 Human
- Entrez Gene: 8361 Human
- Entrez Gene: 8362 Human
- Entrez Gene: 8363 Human
see all -
Alternative names
- dJ160A22.1 antibody
- dJ160A22.2 antibody
- dJ221C16.1 antibody
see all
Images
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Western blot - Anti-Histone H4 (2-hydroxyisobutyryl K16) antibody (ab235006)All lanes : Anti-Histone H4 (2-hydroxyisobutyryl K16) antibody (ab235006) at 1/2000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 5 : HeLa whole cell lysate, untreated
Lane 6 : HEK-293 whole cell lysate, untreated
Lane 7 : A549 whole cell lysate, untreated
Lane 8 : HepG2 whole cell lysate, untreated
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 11 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (2-hydroxyisobutyryl K16) antibody (ab235006)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H4 (2-hydroxyisobutyryl K16) using ab235006 at 1/5 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
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ChIP - Anti-Histone H4 (2-hydroxyisobutyryl K16) antibody (ab235006)HeLa (human epithelial cell line from cervix adenocarcinoma) (4 x 106, treated with 30 mM sodium butyrate for 4 hours) cells were treated with Benzanase, sonicated, and immunoprecipitated with 5 μg ab235006 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
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Immunoprecipitation - Anti-Histone H4 (2-hydroxyisobutyryl K16) antibody (ab235006)Histone H4 (2-hydroxyisobutyryl K16) was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate (treated with 30 mM sodium butyrate for 4 hours) using ab235006 at 1/200 dilution.
Lane 1: Rabbit control IgG in HepG2 whole cell lysate (treated with 30 mM sodium butyrate for 4 hours).
Lane 2: ab235006 IP in HepG2 whole cell lysate (treated with 30 mM sodium butyrate for 4 hours).
Lane 3: HepG2 whole cell lysate (treated with 30 mM sodium butyrate for 4 hours) (20 µg input).
For western blotting an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
References
ab235006 has not yet been referenced specifically in any publications.
