Product nameAnti-Histone H4 (acetyl K12) antibody
See all Histone H4 primary antibodies
DescriptionRabbit polyclonal to Histone H4 (acetyl K12)
Specificityab61238 detects endogenous levels of Histone H4 only when acetylated at lysine 12.
Tested applicationsSuitable for: ELISA, IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human, Drosophila melanogaster
Predicted to work with: Rat
- Human breast carcinoma tissue (IHC-P). Extracts of COS7 cells treated with TSA (400nM, 24hours) (WB).
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab61238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).|
|ICC/IF||1/500 - 1/1000.|
FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similaritiesBelongs to the histone H4 family.
modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- Entrez Gene: 318846 Drosophila melanogaster
- Entrez Gene: 3771854 Drosophila melanogaster
- Entrez Gene: 3771893 Drosophila melanogaster
- Entrez Gene: 3771908 Drosophila melanogaster
- Entrez Gene: 3771935 Drosophila melanogaster
- Entrez Gene: 3771938 Drosophila melanogaster
- Entrez Gene: 3771941 Drosophila melanogaster
- Entrez Gene: 3771947 Drosophila melanogaster
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Histone H4 (acetyl K12) antibody (ab61238) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of acetylated peptide.
All lanes : Anti-Histone H4 (acetyl K12) antibody (ab61238) at 1/500 dilution
Lane 1 : Extracts from COS7 cells, treated
with TSA (400nM, 24hours) with no acetylated peptide
Lane 2 : Extracts from COS7 cells, treated
with TSA (400nM, 24hours) with acetylated peptide
Predicted band size: 11 kDa
Observed band size: 11 kDa
This product has been referenced in:
- de Freitas Filho SAJ et al. Evaluation of specific modified histones in lip carcinogenesis. Pathol Res Pract 214:876-880 (2018). Read more (PubMed: 29699903) »
- Chen D et al. The effects of acetaldehyde exposure on histone modifications and chromatin structure in human lung bronchial epithelial cells. Environ Mol Mutagen 59:375-385 (2018). Read more (PubMed: 29569274) »