Overview

  • Product name

    Anti-Histone H4 (acetyl K12) antibody
    See all Histone H4 primary antibodies
  • Description

    Rabbit polyclonal to Histone H4 (acetyl K12)
  • Host species

    Rabbit
  • Specificity

    ab61238 detects endogenous levels of Histone H4 only when acetylated at lysine 12.
  • Tested applications

    Suitable for: ELISA, IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human, Drosophila melanogaster
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human Histone H4 aa 10-15 (acetyl K12). Synthetic acetylated peptide derived from human Histone H4 around the acetylation site of lysine 12

    LGK(Ac)GG


    Sequence:

    LGK(Ac)GG


    Database link: P62805

  • Positive control

    • Human breast carcinoma tissue (IHC-P). Extracts of COS7 cells treated with TSA (400nM, 24hours) (WB).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
ICC/IF 1/500 - 1/1000.

Target

Images

  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Histone H4 (acetyl K12) antibody (ab61238) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of acetylated peptide.
  • All lanes : Anti-Histone H4 (acetyl K12) antibody (ab61238) at 1/500 dilution

    Lane 1 : Extracts from COS7 cells, treated
    with TSA (400nM, 24hours) with no acetylated peptide
    Lane 2 : Extracts from COS7 cells, treated
    with TSA (400nM, 24hours) with acetylated peptide

    Predicted band size: 11 kDa
    Observed band size: 11 kDa

References

This product has been referenced in:

  • de Freitas Filho SAJ  et al. Evaluation of specific modified histones in lip carcinogenesis. Pathol Res Pract 214:876-880 (2018). Read more (PubMed: 29699903) »
  • Chen D  et al. The effects of acetaldehyde exposure on histone modifications and chromatin structure in human lung bronchial epithelial cells. Environ Mol Mutagen 59:375-385 (2018). Read more (PubMed: 29569274) »
See all 16 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
ChIP
Sample
Human Cell lysate - nuclear (MCF7 breast cancer cell line)
Specification
MCF7 breast cancer cell line
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Positive control
Another company antibody was used

Dr. Sankari Nagarajan

Verified customer

Submitted Apr 05 2019

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15%)
Sample
Human Purified protein (human hepatocarcimona, HepG2)
Specification
human hepatocarcimona, HepG2
Treatment
Various media condition for 24hrs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mr. Chanhee Jo

Verified customer

Submitted Dec 25 2013

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Oocyte)
Specification
Oocyte
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.25% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C

Dr. Maki Asami

Verified customer

Submitted Oct 03 2012

Application
Western blot
Sample
Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (Head)
Loading amount
20 µg
Specification
Head
Gel Running Conditions
Reduced Denaturing (gel %12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Oct 02 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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