Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)
Key features and details
- Rabbit polyclonal to Histone H4 (acetyl K12) - ChIP Grade
- Suitable for: WB, ICC/IF, ChIP
- Reacts with: Human, Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-Histone H4 (acetyl K12) antibody - ChIP Grade
See all Histone H4 primary antibodies -
Description
Rabbit polyclonal to Histone H4 (acetyl K12) - ChIP Grade -
Host species
Rabbit -
Specificity
Ab46983 recognizes Histone H4 acetylated at lysine 12. Serum did not react with non-modified Histone H4 peptide or peptides acetylated at lysines 5, 8 or 16. -
Tested Applications & Species
See all applications and species dataApplication Species ChIP HumanICC/IF HumanWB Human
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Immunogen
This information is considered to be commercially sensitive.
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Positive control
- IF: HeLa cells WB: Acid extract of HeLa cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.035% Sodium azide
Constituent: 30% Glycerol -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab46983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ChIP |
Human
|
| ICC/IF |
Human
|
| WB |
Human
|
| All applications |
a wide range of other species
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/5000 - 1/20000. Predicted molecular weight: 11 kDa.
|
| ICC/IF | (1) |
1/500 - 1/2000.
|
| ChIP | (1) |
Use at an assay dependent concentration. 2 - 8 ul per IP
|
| Notes |
|---|
|
WB
1/5000 - 1/20000. Predicted molecular weight: 11 kDa. |
|
ICC/IF
1/500 - 1/2000. |
|
ChIP
Use at an assay dependent concentration. 2 - 8 ul per IP |
Target
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Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
Sequence similarities
Belongs to the histone H4 family. -
Post-translational
modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 121504 Human
- Entrez Gene: 554313 Human
- Entrez Gene: 8294 Human
- Entrez Gene: 8359 Human
- Entrez Gene: 8360 Human
- Entrez Gene: 8361 Human
- Entrez Gene: 8362 Human
- Entrez Gene: 8363 Human
see all -
Alternative names
- dJ160A22.1 antibody
- dJ160A22.2 antibody
- dJ221C16.1 antibody
see all
Images
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ChIP - Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)
ab46983 tested by ChIP analysis. ChIP performed using Hela chromatin (1.5 x 106 cell equivalents per ChIP) and 10 ul of ab46983 or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as fold enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)Staining of HeLa cells with ab46983 at a dilution of 1/500.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)ab46983 staining Hela cells at 1/500 (top image) and DAPI (bottom image).
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Western blot - Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)All lanes : Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983) at 1/10000 dilution
Lane 1 : Acid extract of
HeLa cells without treatment.
Lane 2 : Acid extract of
HeLa cells treated
with sodium butyrate.
Predicted band size: 11 kDa
Observed band size: 11 kDa
Protocols
References (32)
ab46983 has been referenced in 32 publications.
- He J et al. Transposable elements are regulated by context-specific patterns of chromatin marks in mouse embryonic stem cells. Nat Commun 10:34 (2019). PubMed: 30604769
- Liu W et al. Epigenetic biomarker screening by FLIM-FRET for combination therapy in ER+ breast cancer. Clin Epigenetics 11:16 (2019). PubMed: 30700309
- Guo X et al. HDAC6 is associated with the formation of aortic dissection in human. Mol Med 25:10 (2019). PubMed: 30925865
- Shan C et al. 4-hydroxyphenylpyruvate dioxygenase promotes lung cancer growth via pentose phosphate pathway (PPP) flux mediated by LKB1-AMPK/HDAC10/G6PD axis. Cell Death Dis 10:525 (2019). PubMed: 31285420
- Gruber JJ et al. HAT1 Coordinates Histone Production and Acetylation via H4 Promoter Binding. Mol Cell 75:711-724.e5 (2019). PubMed: 31278053
