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Epigenetics and Nuclear Signaling Histones H3 Acetylated
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Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)

  • Datasheet
  • SDS
Reviews (7) Submit a question References (32)

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ChIP - Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)
  • Western blot - Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)

Key features and details

  • Rabbit polyclonal to Histone H4 (acetyl K12) - ChIP Grade
  • Suitable for: WB, ICC/IF, ChIP
  • Reacts with: Human, Saccharomyces cerevisiae
  • Isotype: IgG

You may also be interested in

Primary
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Anti-Histone H4 (acetyl K8) antibody - ChIP Grade (ab15823)
Protein
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Recombinant Human Histone H4 protein (ab198115)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Histone H4 (acetyl K12) antibody - ChIP Grade
    See all Histone H4 primary antibodies
  • Description

    Rabbit polyclonal to Histone H4 (acetyl K12) - ChIP Grade
  • Host species

    Rabbit
  • Specificity

    Ab46983 recognizes Histone H4 acetylated at lysine 12. Serum did not react with non-modified Histone H4 peptide or peptides acetylated at lysines 5, 8 or 16.
  • Tested Applications & Species

    Application Species
    ChIP
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    This information is considered to be commercially sensitive.

  • Positive control

    • IF: HeLa cells WB: Acid extract of HeLa cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.035% Sodium azide
    Constituent: 30% Glycerol
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Acetylated
    • Epigenetics and Nuclear Signaling
    • Histones
    • H4
    • Acetylated
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies

Associated products

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
  • Recombinant Protein

    • Recombinant Human Histone H4 protein (ab198115)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab46983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ChIP
Human
ICC/IF
Human
WB
Human
All applications
a wide range of other species
Application Abreviews Notes
WB (2)
1/5000 - 1/20000. Predicted molecular weight: 11 kDa.
ICC/IF (1)
1/500 - 1/2000.
ChIP (1)
Use at an assay dependent concentration. 2 - 8 ul per IP
Notes
WB
1/5000 - 1/20000. Predicted molecular weight: 11 kDa.
ICC/IF
1/500 - 1/2000.
ChIP
Use at an assay dependent concentration. 2 - 8 ul per IP

Target

  • Function

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities

    Belongs to the histone H4 family.
  • Post-translational
    modifications

    Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
    Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
    Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
    Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
    Sumoylated, which is associated with transcriptional repression.
  • Cellular localization

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P62805 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 121504 Human
    • Entrez Gene: 554313 Human
    • Entrez Gene: 8294 Human
    • Entrez Gene: 8359 Human
    • Entrez Gene: 8360 Human
    • Entrez Gene: 8361 Human
    • Entrez Gene: 8362 Human
    • Entrez Gene: 8363 Human
    • Entrez Gene: 8364 Human
    • Entrez Gene: 8365 Human
    • Entrez Gene: 8366 Human
    • Entrez Gene: 8367 Human
    • Entrez Gene: 8368 Human
    • Entrez Gene: 8370 Human
    • Omim: 142750 Human
    • Omim: 602822 Human
    • Omim: 602823 Human
    • Omim: 602824 Human
    • Omim: 602825 Human
    • Omim: 602826 Human
    • Omim: 602827 Human
    • Omim: 602828 Human
    • Omim: 602829 Human
    • Omim: 602830 Human
    • Omim: 602831 Human
    • Omim: 602833 Human
    • SwissProt: P62805 Human
    • SwissProt: P02309 Saccharomyces cerevisiae
    • Unigene: 143080 Human
    • Unigene: 247816 Human
    • Unigene: 248172 Human
    • Unigene: 248178 Human
    • Unigene: 248179 Human
    • Unigene: 278483 Human
    • Unigene: 46423 Human
    • Unigene: 528055 Human
    • Unigene: 533295 Human
    • Unigene: 55468 Human
    • Unigene: 591790 Human
    • Unigene: 655235 Human
    • Unigene: 662174 Human
    • Unigene: 706635 Human
    • Unigene: 718935 Human
    see all
  • Alternative names

    • dJ160A22.1 antibody
    • dJ160A22.2 antibody
    • dJ221C16.1 antibody
    • dJ221C16.9 antibody
    • FO108 antibody
    • H4 antibody
    • H4.k antibody
    • H4/a antibody
    • H4/b antibody
    • H4/c antibody
    • H4/d antibody
    • H4/e antibody
    • H4/g antibody
    • H4/h antibody
    • H4/I antibody
    • H4/j antibody
    • H4/k antibody
    • H4/m antibody
    • H4/n antibody
    • H4/p antibody
    • H4_HUMAN antibody
    • H4F2 antibody
    • H4F2iii antibody
    • H4F2iv antibody
    • H4FA antibody
    • H4FB antibody
    • H4FC antibody
    • H4FD antibody
    • H4FE antibody
    • H4FG antibody
    • H4FH antibody
    • H4FI antibody
    • H4FJ antibody
    • H4FK antibody
    • H4FM antibody
    • H4FN antibody
    • H4M antibody
    • HIST1H4A antibody
    • HIST1H4B antibody
    • HIST1H4C antibody
    • HIST1H4D antibody
    • HIST1H4E antibody
    • HIST1H4F antibody
    • HIST1H4H antibody
    • HIST1H4I antibody
    • HIST1H4J antibody
    • HIST1H4K antibody
    • HIST1H4L antibody
    • HIST2H4 antibody
    • HIST2H4A antibody
    • Hist4h4 antibody
    • Histone 1 H4a antibody
    • Histone 1 H4b antibody
    • Histone 1 H4c antibody
    • Histone 1 H4d antibody
    • Histone 1 H4e antibody
    • Histone 1 H4f antibody
    • Histone 1 H4h antibody
    • Histone 1 H4i antibody
    • Histone 1 H4j antibody
    • Histone 1 H4k antibody
    • Histone 1 H4l antibody
    • Histone 2 H4a antibody
    • histone 4 H4 antibody
    • Histone H4 antibody
    • MGC24116 antibody
    see all

Images

  • ChIP - Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)
    ChIP - Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)

    ab46983 tested by ChIP analysis. ChIP performed using Hela chromatin (1.5 x 106 cell equivalents per ChIP) and 10 ul of ab46983 or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as fold enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)

    Staining of HeLa cells with ab46983 at a dilution of 1/500.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)

    ab46983 staining Hela cells at 1/500 (top image) and DAPI (bottom image).

  • Western blot - Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)
    Western blot - Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)
    All lanes : Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983) at 1/10000 dilution

    Lane 1 : Acid extract of
    HeLa cells without treatment.
    Lane 2 : Acid extract of
    HeLa cells treated
    with sodium butyrate.

    Predicted band size: 11 kDa
    Observed band size: 11 kDa

Protocols

  • ChIP protocols
  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (32)

    Publishing research using ab46983? Please let us know so that we can cite the reference in this datasheet.

    ab46983 has been referenced in 32 publications.

    • He J  et al. Transposable elements are regulated by context-specific patterns of chromatin marks in mouse embryonic stem cells. Nat Commun 10:34 (2019). PubMed: 30604769
    • Liu W  et al. Epigenetic biomarker screening by FLIM-FRET for combination therapy in ER+ breast cancer. Clin Epigenetics 11:16 (2019). PubMed: 30700309
    • Guo X  et al. HDAC6 is associated with the formation of aortic dissection in human. Mol Med 25:10 (2019). PubMed: 30925865
    • Shan C  et al. 4-hydroxyphenylpyruvate dioxygenase promotes lung cancer growth via pentose phosphate pathway (PPP) flux mediated by LKB1-AMPK/HDAC10/G6PD axis. Cell Death Dis 10:525 (2019). PubMed: 31285420
    • Gruber JJ  et al. HAT1 Coordinates Histone Production and Acetylation via H4 Promoter Binding. Mol Cell 75:711-724.e5 (2019). PubMed: 31278053
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-7 of 7 Abreviews or Q&A

    ChIP abreview for Anti-Histone H4 (acetyl K12) antibody - ChIP Grade

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (MCF7 breast cancer cell line and tumour samples)
    Specification
    MCF7 breast cancer cell line and tumour samples
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% Formaldehyde
    Positive control
    Another company antibody was used.
    Read More

    Dr. Sankari Nagarajan

    Verified customer

    Submitted Apr 05 2019

    Flow Cytometry abreview for Anti-Histone H4 (acetyl K12) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Zebrafish Cell (Dissociated cells from zebrafish testicle)
    Permeabilization
    Yes - Methanol 90%
    Gating Strategy
    Cell populations were gated according to their DNA content.
    Specification
    Dissociated cells from zebrafish testicle
    Preparation
    Cell harvesting/tissue preparation method: Cells dissociated with collagenase and fixed with paraformaldehyde
    Sample buffer: PBS
    Fixation
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 06 2017

    IHC - Wholemount abreview for Anti-Histone H4 (acetyl K12) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Zebrafish Embryo (Cell nuclei)
    Specification
    Cell nuclei
    Read More

    Marta Lombó Alonso

    Verified customer

    Submitted Sep 06 2017

    Flow Cytometry abreview for Anti-Histone H4 (acetyl K12) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (Thymus)
    Permeabilization
    Yes - FIX & PERM® Cell Fixation & Cell Permeabilization Kit
    Gating Strategy
    Thymocytes (CD45+ Epcam- cells)
    Specification
    Thymus
    Fixation
    FIX & PERM® Cell Fixation & Cell Permeabilization Kit
    Read More

    Abcam user community

    Verified customer

    Submitted May 20 2015

    Western blot abreview for Anti-Histone H4 (acetyl K12) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    2 µg
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Sample
    Xenopus laevis Tissue lysate - whole (Xenopus tectum)
    Specification
    Xenopus tectum
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Dr. Wanhua Shen

    Verified customer

    Submitted Aug 16 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H4 (acetylated) (acetyl K12) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Xenopus laevis Cell (Fibroblast)
    Specification
    Fibroblast
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.05% Triton X-100
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 03 2013

    Western blot abreview for Anti-Histone H4 (acetylated) (acetyl K12) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - nuclear (Mouse Hippocampus)
    Loading amount
    10 µg
    Specification
    Mouse Hippocampus
    Gel Running Conditions
    Reduced Denaturing (4-15% gradient gel)
    Blocking step
    Blocking solution from Roti block as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    Ms. Gonca Bayraktar

    Verified customer

    Submitted Jan 26 2012

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