Product nameAnti-Histone H4 (acetyl K12) antibody - ChIP Grade
See all Histone H4 primary antibodies
DescriptionRabbit polyclonal to Histone H4 (acetyl K12) - ChIP Grade
SpecificityAb46983 recognizes Histone H4 acetylated at lysine 12. Serum did not react with non-modified Histone H4 peptide or peptides acetylated at lysines 5, 8 or 16.
Tested applicationsSuitable for: Dot blot, WB, ICC/IF, ChIP, IPmore details
Species reactivityReacts with: Mouse, Human, Saccharomyces cerevisiae, Xenopus laevis
Predicted to work with: a wide range of other species
This information is considered to be commercially sensitive.
- IF: HeLa cells WB: Acid extract of HeLa cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.035% Sodium azide
Constituent: 30% Glycerol
ChIP Related Products
Our Abpromise guarantee covers the use of ab46983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000. Predicted molecular weight: 11 kDa.|
|ICC/IF||1/500 - 1/2000.|
|ChIP||Use at an assay dependent concentration. 2 - 8 ul per IP|
|IP||Use at an assay dependent concentration.|
FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similaritiesBelongs to the histone H4 family.
modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- dJ160A22.1 antibody
- dJ160A22.2 antibody
- dJ221C16.1 antibody
ab46983 tested by ChIP analysis. ChIP performed using Hela chromatin (1.5 x 106 cell equivalents per ChIP) and 10 ul of ab46983 or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as fold enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
Staining of HeLa cells with ab46983 at a dilution of 1/500.
ab46983 staining Hela cells at 1/500 (top image) and DAPI (bottom image).
All lanes : Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983) at 1/10000 dilution
Lane 1 : Acid extract of
HeLa cells without treatment.
Lane 2 : Acid extract of
HeLa cells treated
with sodium butyrate.
Predicted band size: 11 kDa
Observed band size: 11 kDa
ab46983 in dot blot (top panel), acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed at a dilution of 1:1000. The amount of peptide (picomoles) spotted is shown next to each row.
Lane 1: Histone H4 acetyl-Lys5 peptide.
Lane 2: Unmodified Lys5 peptide.
Lane 3: Acetyl-Lys8 peptide.
Lane 4: Unmodified Lys8 peptide.
Lane 5: Acetyl-Lys12 peptide.
Lane 6: Unmodified Lys12 peptide.
Lane 7: Acetyl-Lys16 peptide.
Lane 8: Unmodified Lys16 peptide.
ab46983 in WB (bottom panel)at 1/2000 with whole-cell extracts (35 ug/lane)from wildtype yeast or yeast containing a mutated histone H4 gene.
Lane 1: Wild-type H4.
Lane 2: H4 N-terminal tail deleted.
Lane 3: Histone H4 K5 to R.
Lane 4: H4 with K8 to R.
Lane 5: H4 with K12 to R.
Lane 6: H4 K16 to R.
Lane 7: H4 K5, 8 and 12 to R.
This product has been referenced in:
- Dannah NS et al. Functional Analysis of Hif1 Histone Chaperone in Saccharomyces cerevisiae. G3 (Bethesda) 8:1993-2006 (2018). Read more (PubMed: 29661843) »
- Ferreira RC et al. Histone Deacetylase 1 Is Essential for Rod Photoreceptor Differentiation by Regulating Acetylation at Histone H3 Lysine 9 and Histone H4 Lysine 12 in the Mouse Retina. J Biol Chem 292:2422-2440 (2017). Read more (PubMed: 28028172) »