Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)
- Datasheet
- References (27)
- Protocols
Overview
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Product name
Anti-Histone H4 (acetyl K12) antibody - ChIP Grade
See all Histone H4 primary antibodies -
Description
Rabbit polyclonal to Histone H4 (acetyl K12) - ChIP Grade -
Host species
Rabbit -
Specificity
Ab46983 recognizes Histone H4 acetylated at lysine 12. Serum did not react with non-modified Histone H4 peptide or peptides acetylated at lysines 5, 8 or 16. -
Tested applications
Suitable for: WB, ICC/IF, ChIPmore details -
Species reactivity
Reacts with: Mouse, Human, Saccharomyces cerevisiae, Xenopus laevis
Predicted to work with: a wide range of other species
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Immunogen
This information is considered to be commercially sensitive.
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Positive control
- IF: HeLa cells WB: Acid extract of HeLa cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.035% Sodium azide
Constituent: 30% Glycerol -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab46983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000 - 1/20000. Predicted molecular weight: 11 kDa. | |
| ICC/IF | 1/500 - 1/2000. | |
| ChIP | Use at an assay dependent concentration. 2 - 8 ul per IP |
Target
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Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
Sequence similarities
Belongs to the histone H4 family. -
Post-translational
modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 121504 Human
- Entrez Gene: 554313 Human
- Entrez Gene: 8294 Human
- Entrez Gene: 8359 Human
- Entrez Gene: 8360 Human
- Entrez Gene: 8361 Human
- Entrez Gene: 8362 Human
- Entrez Gene: 8363 Human
see all -
Alternative names
- dJ160A22.1 antibody
- dJ160A22.2 antibody
- dJ221C16.1 antibody
see all
Images
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ChIP - Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)ab46983 tested by ChIP analysis. ChIP performed using Hela chromatin (1.5 x 106 cell equivalents per ChIP) and 10 ul of ab46983 or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as fold enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)Staining of HeLa cells with ab46983 at a dilution of 1/500.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)ab46983 staining Hela cells at 1/500 (top image) and DAPI (bottom image).
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Western blot - Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)All lanes : Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983) at 1/10000 dilution
Lane 1 : Acid extract of
HeLa cells without treatment.
Lane 2 : Acid extract of
HeLa cells treated
with sodium butyrate.
Predicted band size: 11 kDa
Observed band size: 11 kDa -
Dot Blot - Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)ab46983 in dot blot (top panel), acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed at a dilution of 1:1000. The amount of peptide (picomoles) spotted is shown next to each row.
Lane 1: Histone H4 acetyl-Lys5 peptide.
Lane 2: Unmodified Lys5 peptide.
Lane 3: Acetyl-Lys8 peptide.
Lane 4: Unmodified Lys8 peptide.
Lane 5: Acetyl-Lys12 peptide.
Lane 6: Unmodified Lys12 peptide.
Lane 7: Acetyl-Lys16 peptide.
Lane 8: Unmodified Lys16 peptide.
ab46983 in WB (bottom panel)at 1/2000 with whole-cell extracts (35 ug/lane)from wildtype yeast or yeast containing a mutated histone H4 gene.
Lane 1: Wild-type H4.
Lane 2: H4 N-terminal tail deleted.
Lane 3: Histone H4 K5 to R.
Lane 4: H4 with K8 to R.
Lane 5: H4 with K12 to R.
Lane 6: H4 K16 to R.
Lane 7: H4 K5, 8 and 12 to R.
Protocols
References
This product has been referenced in:
- He J et al. Transposable elements are regulated by context-specific patterns of chromatin marks in mouse embryonic stem cells. Nat Commun 10:34 (2019). Read more (PubMed: 30604769) »
- Liu W et al. Epigenetic biomarker screening by FLIM-FRET for combination therapy in ER+ breast cancer. Clin Epigenetics 11:16 (2019). Read more (PubMed: 30700309) »
