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  1. Link

    histone-h4-acetyl-k16-antibody-epr1004-ab109463.pdf

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Epigenetics and Nuclear Signaling Histones H4 Acetylated
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RecombinantRabMAb

Recombinant Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)

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ChIC/CUT&RUN sequencing - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
  • Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
  • Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
  • Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
  • Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
  • Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
  • Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
  • Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
  • Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
  • Flow Cytometry (Intracellular) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
  • Flow Cytometry (Intracellular) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
  • Flow Cytometry (Intracellular) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
  • Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1004] to Histone H4 (acetyl K16)
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, ChIC/CUT&RUN-seq
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free HRP

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Overview

  • Product name

    Anti-Histone H4 (acetyl K16) antibody [EPR1004]
    See all Histone H4 primary antibodies
  • Description

    Rabbit monoclonal [EPR1004] to Histone H4 (acetyl K16)
  • Host species

    Rabbit
  • Specificity

    ab109463 only detects Histone H4 acetylated on Lysine 16.
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, ChIC/CUT&RUN-seqmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, C6 and mouse spleen cell lysates - treated with TSA. IHC-P: Human testis, transitional cell carcinoma and colon tissues. ICC/IF: HeLa cells treated with TSA. Flow Cyt (intra): HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1004
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H4
    • Acetylated
    • Epigenetics and Nuclear Signaling
    • Histones
    • H4
    • Ubiquitinated
    • Epigenetics and Nuclear Signaling
    • Histones
    • H4
    • Other Modifications

Associated products

  • Alternative Versions

    • Anti-Histone H4 (acetyl K16) antibody [EPR1004] - BSA and Azide free (ab194352)
    • HRP Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab200859)
    • Alexa Fluor® 488 Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab246771)
    • Alexa Fluor® 647 Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab246772)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Mouse spleen tissue lysate - total protein (ab29293)
    • Mouse spleen tissue lysate - total protein (14 days) (ab7274)
    • Mouse spleen tissue lysate - total protein (ab7937)
  • Recombinant Protein

    • Recombinant Human Histone H4 protein (ab198115)
  • Related Products

    • Human Histone H4 peptide (ab13843)
    • Human Histone H4 (symmetric di methyl R3) peptide (ab14791)
    • Human Histone H4 (tri methyl K20) peptide (ab17567)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/100 - 1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (4)
1/1000 - 1/2000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
IHC-P (2)
1/100 - 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

ICC/IF (1)
1/100 - 1/200.
ChIC/CUT&RUN-seq
Use µg for 105 cells.
Notes
Flow Cyt (Intra)
1/100 - 1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/2000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
IHC-P
1/100 - 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

ICC/IF
1/100 - 1/200.
ChIC/CUT&RUN-seq
Use µg for 105 cells.
Application notes
Is unsuitable for IP.

Target

  • Function

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities

    Belongs to the histone H4 family.
  • Post-translational
    modifications

    Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
    Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
    Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
    Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
    Sumoylated, which is associated with transcriptional repression.
  • Cellular localization

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P62805 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 121504 Human
    • Entrez Gene: 554313 Human
    • Entrez Gene: 8294 Human
    • Entrez Gene: 8359 Human
    • Entrez Gene: 8360 Human
    • Entrez Gene: 8361 Human
    • Entrez Gene: 8362 Human
    • Entrez Gene: 8363 Human
    • Entrez Gene: 8364 Human
    • Entrez Gene: 8365 Human
    • Entrez Gene: 8366 Human
    • Entrez Gene: 8367 Human
    • Entrez Gene: 8368 Human
    • Entrez Gene: 8370 Human
    • Entrez Gene: 100041230 Mouse
    • Entrez Gene: 319155 Mouse
    • Entrez Gene: 319156 Mouse
    • Entrez Gene: 319157 Mouse
    • Entrez Gene: 319158 Mouse
    • Entrez Gene: 319159 Mouse
    • Entrez Gene: 319160 Mouse
    • Entrez Gene: 319161 Mouse
    • Entrez Gene: 320332 Mouse
    • Entrez Gene: 326619 Mouse
    • Entrez Gene: 326620 Mouse
    • Entrez Gene: 674678 Mouse
    • Entrez Gene: 69386 Mouse
    • Entrez Gene: 97122 Mouse
    • Entrez Gene: 64627 Rat
    • Omim: 142750 Human
    • Omim: 602822 Human
    • Omim: 602823 Human
    • Omim: 602824 Human
    • Omim: 602825 Human
    • Omim: 602826 Human
    • Omim: 602827 Human
    • Omim: 602828 Human
    • Omim: 602829 Human
    • Omim: 602830 Human
    • Omim: 602831 Human
    • Omim: 602833 Human
    • SwissProt: P62805 Human
    • SwissProt: P62806 Mouse
    • SwissProt: P62804 Rat
    • Unigene: 143080 Human
    • Unigene: 247816 Human
    • Unigene: 248172 Human
    • Unigene: 248178 Human
    • Unigene: 248179 Human
    • Unigene: 278483 Human
    • Unigene: 46423 Human
    • Unigene: 528055 Human
    • Unigene: 533295 Human
    • Unigene: 55468 Human
    • Unigene: 591790 Human
    • Unigene: 655235 Human
    • Unigene: 662174 Human
    • Unigene: 706635 Human
    • Unigene: 718935 Human
    • Unigene: 144300 Mouse
    • Unigene: 14775 Mouse
    • Unigene: 158272 Mouse
    • Unigene: 227295 Mouse
    • Unigene: 228709 Mouse
    • Unigene: 246720 Mouse
    • Unigene: 255646 Mouse
    • Unigene: 260530 Mouse
    • Unigene: 261642 Mouse
    • Unigene: 261662 Mouse
    • Unigene: 261664 Mouse
    • Unigene: 377875 Mouse
    • Unigene: 442307 Mouse
    see all
  • Alternative names

    • dJ160A22.1 antibody
    • dJ160A22.2 antibody
    • dJ221C16.1 antibody
    • dJ221C16.9 antibody
    • FO108 antibody
    • H4 antibody
    • H4.k antibody
    • H4/a antibody
    • H4/b antibody
    • H4/c antibody
    • H4/d antibody
    • H4/e antibody
    • H4/g antibody
    • H4/h antibody
    • H4/I antibody
    • H4/j antibody
    • H4/k antibody
    • H4/m antibody
    • H4/n antibody
    • H4/p antibody
    • H4_HUMAN antibody
    • H4F2 antibody
    • H4F2iii antibody
    • H4F2iv antibody
    • H4FA antibody
    • H4FB antibody
    • H4FC antibody
    • H4FD antibody
    • H4FE antibody
    • H4FG antibody
    • H4FH antibody
    • H4FI antibody
    • H4FJ antibody
    • H4FK antibody
    • H4FM antibody
    • H4FN antibody
    • H4M antibody
    • HIST1H4A antibody
    • HIST1H4B antibody
    • HIST1H4C antibody
    • HIST1H4D antibody
    • HIST1H4E antibody
    • HIST1H4F antibody
    • HIST1H4H antibody
    • HIST1H4I antibody
    • HIST1H4J antibody
    • HIST1H4K antibody
    • HIST1H4L antibody
    • HIST2H4 antibody
    • HIST2H4A antibody
    • Hist4h4 antibody
    • Histone 1 H4a antibody
    • Histone 1 H4b antibody
    • Histone 1 H4c antibody
    • Histone 1 H4d antibody
    • Histone 1 H4e antibody
    • Histone 1 H4f antibody
    • Histone 1 H4h antibody
    • Histone 1 H4i antibody
    • Histone 1 H4j antibody
    • Histone 1 H4k antibody
    • Histone 1 H4l antibody
    • Histone 2 H4a antibody
    • histone 4 H4 antibody
    • Histone H4 antibody
    • MGC24116 antibody
    see all

Images

  • ChIC/CUT&RUN sequencing - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    ChIC/CUT&RUN sequencing - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)

    CUT&RUN was performed using the ChIC/CUT&RUN pAG-MNAse ab285373 105 HeLa cells and 2µg of ab109463 [EPR1004]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. 

    Additional screenshots of mapped reads can be downloaded here. 

  • Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Lanes 1-2 : Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463) at 1/6000 dilution
    Lanes 3-4 : Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463) at 1/24000 dilution

    Lanes 1 & 3 : Untreated C6 (Rat glial tumor glial cell) whole cell lysate
    Lanes 2 & 4 : C6 (Rat glial tumor glial cell) treated with Trichostatin A (final concentration is 500ng/ml) for 4 hours whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 11 kDa


    Exposure time: 3 minutes


    Blocking/Diluting buffer and concentration 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)

    Immunocytochemistry/ Immunofluorescence analysis of untreated HeLa cells (top row) and HeLa+ TSA(500ng/ml, 4h) cells (middle row) labeling Histone H4 (acetyl K16) with ab109463 at 1/500. Goat anti rabbit IgG(Alexa Fluor® 488); ab150077 at 1/1000 dilution was used as the secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilised with 0.1% tritonX-100. DAPI (blue) was used as a nuclear counterstain.



  • Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    All lanes : Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysates, untreated
    Lane 2 : HeLa cell lysates treated with TSA

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa

  • Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463) at 1/1000 dilution (unpurified) + HeLa cell lysate - treated with TSA at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463) at 1/1500 dilution (purified) + HeLa cell lysate - treated with TSA at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463) at 1/1000 dilution (unpurified) + C6 cell lysate - treated with TSA at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463) at 1/1500 dilution (unpurified) + C6 cell lysate - treated with TSA at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463) at 1/1000 dilution (purified) + Mouse spleen tissue lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L)

    Predicted band size: 11 kDa
    Observed band size: 11 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463) at 1/1500 dilution (purified) + Mouse spleen tissue lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Histone H4 (acetyl K16) with unpurified ab109463 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Histone H4 (acetyl K16) with purified ab109463 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling Histone H4 with unpurified ab109463 at 1/100.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human transitional cell carcinoma labelling Histone H4 (acetly K16) with unpurified ab109463 at 1/100.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Histone H4 (acetyl K16) with unpurified ab109463 (red) at 1/100. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/100) and secondary antibody ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells treated with TSA labelling Histone H4 (acetyl K16) with unpurified ab109463 (red) at 1/100. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/100) and secondary antibody ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Histone H4 (acetyl K16) with purified ab109463 (red) at 1/150. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/150) and secondary antibody ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells treated with TSA labelling Histone H4 (acetyl K16) with purified ab109463 (red) at 1/150. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/150) and secondary antibody ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

  • Flow Cytometry (Intracellular) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Flow Cytometry (Intracellular) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)

    Overlay histogram showing HeLa cells stained with unpurified ab109463 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109463, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluorr® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. 

     

     

  • Flow Cytometry (Intracellular) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Flow Cytometry (Intracellular) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)

    Intracellular Flow Cytometry analysis of HeLa cells labelling Histone H4 (acetyl K16)with unpurified ab109463 (red) at 1/130. Cells were fixed with 80% methanol. A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/150). A rabbit monoclonal IgG was used as the isotype control (green). 

     

     

  • Flow Cytometry (Intracellular) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Flow Cytometry (Intracellular) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)

    Intracellular Flow Cytometry analysis of HeLa cells labelling Histone H4 (acetyl K16) with purified ab109463 (red) at 1/200. Cells were fixed with 80% methanol. A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/150). A rabbit monoclonal IgG was used as the isotype control (green). 

     

     

  • Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
    Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols
  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

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References (58)

Publishing research using ab109463? Please let us know so that we can cite the reference in this datasheet.

ab109463 has been referenced in 58 publications.

  • Song Z  et al. Effects of histone H4 hyperacetylation on inhibiting MMP2 and MMP9 in human amniotic epithelial cells and in premature rupture of fetal membranes. Exp Ther Med 21:515 (2021). PubMed: 33815588
  • Shalini V  et al. Genome-wide occupancy reveals the localization of H1T2 (H1fnt) to repeat regions and a subset of transcriptionally active chromatin domains in rat spermatids. Epigenetics Chromatin 14:3 (2021). PubMed: 33407810
  • Contreras SM  et al. Resveratrol induces H3 and H4K16 deacetylation and H2A.X phosphorylation in Toxoplasma gondii. BMC Res Notes 14:19 (2021). PubMed: 33413578
  • Huang R  et al. HDAC11 inhibition disrupts porcine oocyte meiosis via regulating a-tubulin acetylation and histone modifications. Aging (Albany NY) 13:8849-8864 (2021). PubMed: 33742608
  • Navarro-Carrasco E & Lazo PA VRK1 Depletion Facilitates the Synthetic Lethality of Temozolomide and Olaparib in Glioblastoma Cells. Front Cell Dev Biol 9:683038 (2021). PubMed: 34195200
View all Publications for this product

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1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H4 (acetyl K16) antibody [EPR1004]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colorectal cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization
Yes - PBS-Tween
Specification
Colorectal cancer
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Formaldehyde
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Abcam user community

Verified customer

Submitted Jul 23 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H4 (acetyl K16) antibody [EPR1004]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Na-citrate pH6
Sample
Mouse Tissue sections (Liver, P5)
Specification
Liver, P5
Permeabilization
Yes - Triton 0,05%
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Oct 07 2014

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