Recombinant
RabMAb

Recombinant Anti-Histone H4 (acetyl K16) antibody [EPR1004] (HRP) (ab200859)

Overview

  • Product name

    Anti-Histone H4 (acetyl K16) antibody [EPR1004] (HRP)
    See all Histone H4 primary antibodies
  • Description

    Rabbit monoclonal [EPR1004] to Histone H4 (acetyl K16) (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Histone H4 (acetyl K16). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa TSA Treated whole cell lysate. IHC-P: FFPE Human Colon (Normal) tissue slides.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.4
    Preservative: 0.1% Proclin
    Constituents: 30% Glycerol, PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1004
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab200859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

Images

  • All lanes : Anti-Histone H4 (acetyl K16) antibody [EPR1004] (HRP) (ab200859) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate TSA Treated

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 14 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab200859 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • IHC image of Histone H4 (acetyl K16) staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with Tris EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab200859, 1/50 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References

ab200859 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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