Recombinant
RabMAb

Anti-Histone H4 (acetyl K20) antibody [EPR16998(2)] (ab177191)

Overview

  • Product name
    Anti-Histone H4 (acetyl K20) antibody [EPR16998(2)]
    See all Histone H4 primary antibodies
  • Description
    Rabbit monoclonal [EPR16998(2)] to Histone H4 (acetyl K20)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Histone H4 aa 1-100 (acetyl K20). The exact sequence is proprietary.
    Database link: P62805

  • Positive control
    • WB: HeLa whole cell lysates untreated and treated with 500 ng/ml TSA for 4 hours; NIH/3T3 whole cell lysates untreated and treated with 500 ng/ml TSA for 4 hours. ICC/IF: HeLa and NIH/3T3 cells treated with Trichostatin A (500 ng/ml) for 4 hours.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR16998(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
WB 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).

Target

  • Function
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities
    Belongs to the histone H4 family.
  • Post-translational
    modifications
    Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
    Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
    Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
    Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
    Sumoylated, which is associated with transcriptional repression.
  • Cellular localization
    Nucleus. Chromosome.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Histone gene cluster 1, H4A antibody
    • Histone gene cluster 2, H4 antibody
    • dJ160A22.1 antibody
    • dJ160A22.2 antibody
    • dJ221C16.1 antibody
    • dJ221C16.9 antibody
    • FO108 antibody
    • H4 antibody
    • H4 histone family, member A antibody
    • H4 histone family, member B antibody
    • H4 histone family, member C antibody
    • H4 histone family, member D antibody
    • H4 histone family, member E antibody
    • H4 histone family, member G antibody
    • H4 histone family, member H antibody
    • H4 histone family, member I antibody
    • H4 histone family, member J antibody
    • H4 histone family, member K antibody
    • H4 histone family, member M antibody
    • H4 histone family, member N antibody
    • H4 histone, family 2 antibody
    • H4/A antibody
    • H4/B antibody
    • H4/C antibody
    • H4/D antibody
    • H4/E antibody
    • H4/G antibody
    • H4/H antibody
    • H4/I antibody
    • H4/J antibody
    • H4/K antibody
    • H4/M antibody
    • H4/N antibody
    • H4/O antibody
    • H4/p antibody
    • H4_HUMAN antibody
    • H4F2 antibody
    • H4F2iii antibody
    • H4F2iv antibody
    • H4FA antibody
    • H4FB antibody
    • H4FC antibody
    • H4FD antibody
    • H4FE antibody
    • H4FG antibody
    • H4FH antibody
    • H4FI antibody
    • H4FJ antibody
    • H4FK antibody
    • HIST1 cluster, H4A antibody
    • HIST1 cluster, H4B antibody
    • HIST1 cluster, H4D antibody
    • HIST2H4 antibody
    • Hist4 cluster, H4 antibody
    • Hist4h4 antibody
    • histone 1, H4a antibody
    • histone 1, H4c antibody
    • histone 1, H4d antibody
    • histone 1, H4f antibody
    • histone 1, H4h antibody
    • histone 1, H4i antibody
    • histone 1, H4j antibody
    • histone 1, H4k antibody
    • histone 1, H4l antibody
    • histone 2, H4a antibody
    • histone 2, H4b antibody
    • Histone 4 family, member M antibody
    • histone 4, H4 antibody
    • histone cluster 1, H4 antibody
    • histone cluster 1, H4a antibody
    • histone cluster 1, H4b antibody
    • histone cluster 1, H4c antibody
    • histone cluster 1, H4d antibody
    • histone cluster 1, H4e antibody
    • histone cluster 1, H4f antibody
    • histone cluster 1, H4h antibody
    • histone cluster 1, H4i antibody
    • histone cluster 1, H4j antibody
    • histone cluster 1, H4k antibody
    • histone cluster 1, H4l antibody
    • histone cluster 2, H4a antibody
    • histone cluster 2, H4b antibody
    • histone cluster 4, H4 antibody
    • Histone family, member A antibody
    • Histone family, member B antibody
    • Histone family, member D antibody
    • Histone family, member H antibody
    • Histone family, member I antibody
    • Histone family, member L antibody
    • Histone gene cluster 1, H4 antibody
    • Histone gene cluster 1, H4D antibody
    • Histone gene cluster 1, H4E antibody
    • Histone gene cluster 1, H4K antibody
    • Histone gene cluster 4, H4 antibody
    • Histone gene cluster 4, H4 histone antibody
    • Histone H4 antibody
    • histone IV, family 2 antibody
    see all

Images

  • All lanes : Anti-Histone H4 (acetyl K20) antibody [EPR16998(2)] (ab177191) at 1/1000 dilution

    Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 500 ng/ml TSA for 4 hours

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Histone H4 (acetyl K20) antibody [EPR16998(2)] (ab177191) at 1000 cells

    Lane 1 : Untreated NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate treated with 500 ng/ml TSA for 4 hours

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Histone H4 (acetyl K20) antibody [EPR16998(2)] (ab177191) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 500 ng/ml TSA for 4 hours
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 500 ng/ml TSA for 4 hours with H4K20ac peptide
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 500 ng/ml TSA for 4 hours with H4K20 unmodified peptide
    Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 500 ng/ml TSA for 4 hours with H4K16Me1 peptide
    Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 500 ng/ml TSA for 4 hours with H4K16ac peptide
    Lane 6 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 500 ng/ml TSA for 4 hours with H4K16 unmodified peptide

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    5 μg peptide is used in each lane of the blocking experiment

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H4 (acetyl K20) with ab177191 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    The expression increased on HeLa cells after treatment with Trichostatin A (500 ng/ml) for 4 hours.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab177191 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling Histone H4 (acetyl K20) with ab177191 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    The expression increased on NIH/3T3 cells after treatment with Trichostatin A (500 ng/ml) for 4 hours.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab177191 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

References

ab177191 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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