Overview

  • Product name

    Anti-Histone H4 (acetyl K5) antibody
    See all Histone H4 primary antibodies
  • Description

    Rabbit polyclonal to Histone H4 (acetyl K5)
  • Host species

    Rabbit
  • Specificity

    ab124636 detects endogenous levels of total Histone H4 protein only when acetylated at Lysine 5.
  • Tested applications

    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Histone H4 (acetyl K5). A synthetic peptide from human Histone H4 around the acetylated site of Lys5.
    Database link: P62805

  • Positive control

    • Human prostate, COS7 cells, HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.9% Sodium chloride
    Note: without Mg2+, Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 11 kDa.
ELISA 1/1000.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF 1/100 - 1/500.

Target

Images

  • All lanes : Anti-Histone H4 (acetyl K5) antibody (ab124636) at 1/500 dilution

    Lane 1 : COS7 cell extract, treated with TSA 400nM 24h.
    Lane 2 : COS7 cell extract, treated with TSA 400nM 24h. with synthesized peptide

    Predicted band size: 11 kDa

  • ab124636, at 5µg/mL, staining Human prostate by immunohistochemistry of paraffin embedded tissues (IHC-P).
  • ab124636, at 1/100 dilution, staining HeLa cells, by immunofluorescence. The picture on the right is treated with synthesized peptide.

References

This product has been referenced in:

See 1 Publication for this product

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