Key features and details
- Rabbit polyclonal to Histone H4 (acetyl K5)
- Suitable for: WB, ELISA, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Histone H4 (acetyl K5) antibody
See all Histone H4 primary antibodies
DescriptionRabbit polyclonal to Histone H4 (acetyl K5)
Specificityab124636 detects endogenous levels of total Histone H4 protein only when acetylated at Lysine 5.
Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human Histone H4 (acetyl K5). A synthetic peptide from human Histone H4 around the acetylated site of Lys5.
Database link: P62805
- Human prostate, COS7 cells, HeLa cells.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.9% Sodium chloride, 50% Glycerol
without Mg2+, Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab124636 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 11 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||1/100 - 1/500.|
FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similaritiesBelongs to the histone H4 family.
modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
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All lanes : Anti-Histone H4 (acetyl K5) antibody (ab124636) at 1/500 dilution
Lane 1 : COS7 cell extract, treated with TSA 400nM 24h.
Lane 2 : COS7 cell extract, treated with TSA 400nM 24h. with synthesized peptide
Predicted band size: 11 kDa
ab124636, at 5µg/mL, staining Human prostate by immunohistochemistry of paraffin embedded tissues (IHC-P).
ab124636, at 1/100 dilution, staining HeLa cells, by immunofluorescence. The picture on the right is treated with synthesized peptide.
ab124636 has been referenced in 2 publications.
- Li D et al. Identification of histone acetylation markers in human fetal brains and increased H4K5ac expression in neural tube defects. Mol Genet Genomic Med 7:e1002 (2019). PubMed: 31612645
- Fang L et al. Mechanisms Underlying Acrolein-Mediated Inhibition of Chromatin Assembly. Mol Cell Biol 36:2995-3008 (2016). PubMed: 27669733