Histone H4 (acetyl K5) Quantification Kit (Colorimetric) (ab115116)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameHistone H4 (acetyl K5) Quantification Kit (Colorimetric)
See all Histone H4 (K5 acetylation) kits -
Sample typeTissue, Adherent cells, Suspension cells
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Sensitivity2 ng/well
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Range20 ng/well - 5000 ng/well
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Assay time2h 30m
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Mammals
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Product overview
Acetylation of histones such histone H4 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H4 actylation. Histone H4 (acetyl K5) reflects the hyperacetylated stated in histone H4 and strongly correlates with active state of genes. H4 (acetyl K5) is also considered to be a marker of dexamethasone transactivation and a change in H4 (acetyl K5) is observed in cancer and inflammatory diseases.
Abcam's Histone H4 (acetyl K5) Quantification Kit (Colorimetric) (ab115116) allows the experimenter to measure global acetylation of histone H4K5 quickly and consistently. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment and it is suitable for specifically measuring global histone H4K5 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
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Notes
Properties
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Storage instructionsPlease refer to protocols.
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Components Identifier 48 tests 96 tests 10X Wash Buffer 1 x 10ml 1 x 20ml 8-Well Sample Strips (with Frame) 4 units 9 units 8-Well Standard Control Strips Green Ringed 2 units 3 units Antibody Buffer 1 x 6ml 1 x 12ml Color Developer 1 x 5ml 1 x 10ml Detection Antibody, 1 mg/mL 1 x 5µl 1 x 10µl Signal Enhancer 1 x 120µl 1 x 240µl Signal Report Solution 1 x 5µl 1 x 10µl Standard Control, 100 µg/mL 1 x 10µl 1 x 20µl Stop Solution 1 x 3ml 1 x 6ml -
Research areas
Associated products
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Related Products
- Histone Extraction Kit (ab113476)
- Histone H4 (acetyl K5) Quantification Kit (Fluorometric) (ab115117)
- Histone H4 (acetyl K8) Quantification Kit (Colorimetric) (ab115118)
- Histone H4 (acetyl K8) Quantification Kit (Fluorometric) (ab115119)
- Histone H4 (acetyl K16) Quantification Kit (Colorimetric) (ab115120)
- Histone H4 (acetyl K16) Quantification Kit (Fluorometric) (ab115121)
- Histone H4 (acetyl K12) Quantification Kit (Colorimetric) (ab115122)
- Histone H4 (acetyl K12) Quantification Kit (Fluorometric) (ab115123)
Protocols
References
This product has been referenced in:
- Jha PK et al. HAT2 mediates histone H4K4 acetylation and affects micrococcal nuclease sensitivity of chromatin in Leishmania donovani. PLoS One 12:e0177372 (2017). Leishmania . Read more (PubMed: 28486547) »
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