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  1. Link

    histone-h4-acetyl-k5-quantification-kit-colorimetric-ab115116.pdf

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Epigenetics and Nuclear Signaling Histones H4 Acetylated
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Histone H4 (acetyl K5) Quantification Kit (Colorimetric) (ab115116)

  • Datasheet
  • SDS
  • Protocol Booklet
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Key features and details

  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Assay time: 2 hr 30 min
  • Sample type: Adherent cells, Suspension cells, Tissue
  • Sensitivity: 2 ng/well

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Overview

  • Product name

    Histone H4 (acetyl K5) Quantification Kit (Colorimetric)
    See all Histone H4 kits
  • Detection method

    Colorimetric
  • Sample type

    Tissue, Adherent cells, Suspension cells
  • Sensitivity

    2 ng/well
  • Range

    20 ng/well - 5000 ng/well
  • Assay time

    2h 30m
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Mammals
  • Product overview

    Acetylation of histones such histone H4 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H4 actylation. Histone H4 (acetyl K5) reflects the hyperacetylated stated in histone H4 and strongly correlates with active state of genes. H4 (acetyl K5) is also considered to be a marker of dexamethasone transactivation and a change in H4 (acetyl K5) is observed in cancer and inflammatory diseases.

    Abcam's Histone H4 (acetyl K5) Quantification Kit (Colorimetric) (ab115116) allows the experimenter to measure global acetylation of histone H4K5 quickly and consistently. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment and it is suitable for specifically measuring global histone H4K5 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.

  • Notes

     

     

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Please refer to protocols.
  • Components Identifier 48 tests 96 tests
    10X Wash Buffer 1 x 10ml 1 x 20ml
    8-Well Sample Strips (with Frame) 4 units 9 units
    8-Well Standard Control Strips Green Ringed 2 units 3 units
    Antibody Buffer 1 x 6ml 1 x 12ml
    Color Developer 1 x 5ml 1 x 10ml
    Detection Antibody, 1 mg/mL 1 x 5µl 1 x 10µl
    Signal Enhancer 1 x 120µl 1 x 240µl
    Signal Report Solution 1 x 5µl 1 x 10µl
    Standard Control, 100 µg/mL 1 x 10µl 1 x 20µl
    Stop Solution 1 x 3ml 1 x 6ml
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H4
    • Acetylated
    • Kits/ Lysates/ Other
    • Kits
    • Epigenetic kits
    • Histone acetylation and deacetylation
    • Epigenetics and Nuclear Signaling
    • Assays & Kits
    • Histone acetylation and deacetylation
  • Function

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities

    Belongs to the histone H4 family.
  • Post-translational
    modifications

    Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
    Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
    Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
    Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
    Sumoylated, which is associated with transcriptional repression.
  • Cellular localization

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P62805 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • dJ160A22.1
    • dJ160A22.2
    • dJ221C16.1
    • dJ221C16.9
    • FO108
    • H4
    • H4.k
    • H4/a
    • H4/b
    • H4/c
    • H4/d
    • H4/e
    • H4/g
    • H4/h
    • H4/I
    • H4/j
    • H4/k
    • H4/m
    • H4/n
    • H4/p
    • H4_HUMAN
    • H4F2
    • H4F2iii
    • H4F2iv
    • H4FA
    • H4FB
    • H4FC
    • H4FD
    • H4FE
    • H4FG
    • H4FH
    • H4FI
    • H4FJ
    • H4FK
    • H4FM
    • H4FN
    • H4M
    • HIST1H4A
    • HIST1H4B
    • HIST1H4C
    • HIST1H4D
    • HIST1H4E
    • HIST1H4F
    • HIST1H4H
    • HIST1H4I
    • HIST1H4J
    • HIST1H4K
    • HIST1H4L
    • HIST2H4
    • HIST2H4A
    • Hist4h4
    • Histone 1 H4a
    • Histone 1 H4b
    • Histone 1 H4c
    • Histone 1 H4d
    • Histone 1 H4e
    • Histone 1 H4f
    • Histone 1 H4h
    • Histone 1 H4i
    • Histone 1 H4j
    • Histone 1 H4k
    • Histone 1 H4l
    • Histone 2 H4a
    • histone 4 H4
    • Histone H4
    • MGC24116
    see all
  • Database links

    • Entrez Gene: 121504 Human
    • Entrez Gene: 554313 Human
    • Entrez Gene: 8294 Human
    • Entrez Gene: 8359 Human
    • Entrez Gene: 8360 Human
    • Entrez Gene: 8361 Human
    • Entrez Gene: 8368 Human
    • Entrez Gene: 8370 Human
    • Entrez Gene: 8362 Human
    • Entrez Gene: 8363 Human
    • Entrez Gene: 8364 Human
    • Entrez Gene: 8365 Human
    • Entrez Gene: 8366 Human
    • Entrez Gene: 8367 Human
    • Entrez Gene: 100041230 Mouse
    • Entrez Gene: 319155 Mouse
    • Entrez Gene: 319156 Mouse
    • Entrez Gene: 319157 Mouse
    • Entrez Gene: 319158 Mouse
    • Entrez Gene: 319159 Mouse
    • Entrez Gene: 69386 Mouse
    • Entrez Gene: 97122 Mouse
    • Entrez Gene: 319160 Mouse
    • Entrez Gene: 319161 Mouse
    • Entrez Gene: 320332 Mouse
    • Entrez Gene: 326619 Mouse
    • Entrez Gene: 326620 Mouse
    • Entrez Gene: 674678 Mouse
    • Omim: 142750 Human
    • Omim: 602822 Human
    • Omim: 602829 Human
    • Omim: 602827 Human
    • Omim: 602823 Human
    • Omim: 602830 Human
    • Omim: 602824 Human
    • Omim: 602828 Human
    • Omim: 602833 Human
    • Omim: 602826 Human
    • Omim: 602825 Human
    • Omim: 602831 Human
    • SwissProt: P62805 Human
    • SwissProt: P62806 Mouse
    • Unigene: 143080 Human
    • Unigene: 247816 Human
    • Unigene: 248172 Human
    • Unigene: 248178 Human
    • Unigene: 248179 Human
    • Unigene: 278483 Human
    • Unigene: 662174 Human
    • Unigene: 706635 Human
    • Unigene: 718935 Human
    • Unigene: 46423 Human
    • Unigene: 528055 Human
    • Unigene: 533295 Human
    • Unigene: 55468 Human
    • Unigene: 591790 Human
    • Unigene: 655235 Human
    • Unigene: 144300 Mouse
    • Unigene: 14775 Mouse
    • Unigene: 158272 Mouse
    • Unigene: 227295 Mouse
    • Unigene: 228709 Mouse
    • Unigene: 246720 Mouse
    • Unigene: 442307 Mouse
    • Unigene: 255646 Mouse
    • Unigene: 260530 Mouse
    • Unigene: 261642 Mouse
    • Unigene: 261662 Mouse
    • Unigene: 261664 Mouse
    • Unigene: 377875 Mouse
    see all

Associated products

  • Related Products

    • Histone Extraction Kit (ab113476)
    • Histone H4 (acetyl K5) Quantification Kit (Fluorometric) (ab115117)
    • Histone H4 (acetyl K8) Quantification Kit (Colorimetric) (ab115118)
    • Histone H4 (acetyl K8) Quantification Kit (Fluorometric) (ab115119)
    • Histone H4 (acetyl K16) Quantification Kit (Colorimetric) (ab115120)
    • Histone H4 (acetyl K16) Quantification Kit (Fluorometric) (ab115121)
    • Histone H4 (acetyl K12) Quantification Kit (Colorimetric) (ab115122)
    • Histone H4 (acetyl K12) Quantification Kit (Fluorometric) (ab115123)

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab115116? Please let us know so that we can cite the reference in this datasheet.

ab115116 has been referenced in 1 publication.

  • Jha PK  et al. HAT2 mediates histone H4K4 acetylation and affects micrococcal nuclease sensitivity of chromatin in Leishmania donovani. PLoS One 12:e0177372 (2017). Leishmania . PubMed: 28486547

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