Key features and details
- Rabbit polyclonal to Histone H4 (acetyl K8)
- Suitable for: WB, IP, IHC-P, ChIP, Dot blot, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Histone H4 (acetyl K8) antibody
See all Histone H4 primary antibodies
DescriptionRabbit polyclonal to Histone H4 (acetyl K8)
Tested applicationsSuitable for: WB, IP, IHC-P, ChIP, Dot blot, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Xenopus laevis
Synthetic peptide within Human Histone H4 (N terminal) (acetyl K8). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: P62805
- ICC/IF: HeLa cells. IHC-P: Rat hind brain tissue. Mouse kidney and duodenum tissue. WB: HeLa treated with DMSO or Trichostatin A, whole cell lysates. IP: HeLa, treated with TSA, whole cell lysate. ChIP: HeLa chromatin extract treated with Trichostatin A.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227855 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 11 kDa.|
|IP||1/100 - 1/5000.|
|IHC-P||1/100 - 1/1000.|
|ChIP||Use at an assay dependent concentration.|
|Dot blot||Use at an assay dependent concentration.|
|ICC/IF||1/100 - 1/1000.|
FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similaritiesBelongs to the histone H4 family.
modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- dJ160A22.1 antibody
- dJ160A22.2 antibody
- dJ221C16.1 antibody
All lanes : Anti-Histone H4 (acetyl K8) antibody (ab227855) at 1/5000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract treated with 0.012% DMSO for 18 hours
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract treated with 0.4 µM Trichostatin A for 18 hours
Lysates/proteins at 30 µg per lane.
Predicted band size: 11 kDa
15% SDS-PAGE gel.
4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H4 (acetyl K8) (green) using ab227855 at 1/500 dilution in ICC/IF.
Red: alpha Tubulin stained with an alpha Tubulin antibody at 1/500 dilution.
Blue: Hoechst 33342 staining.
Cross-linked ChIP was performed with HeLa (human epithelial cell line from cervix adenocarcinoma) chromatin extract treated with Trichostatin A (0.4 μM for 18 hours) and 5 μg of either control rabbit IgG or ab227855. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or TEXT12.
Histone H4 (acetyl K8) was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 2.5 µg ab227855. Western blot was performed from the immunoprecipitate using ab227855. Anti-rabbit IgG (HRP) was used as a secondary reagent.
Lane 1: 30 μg whole cell lysate/extract of Histone H4 (acetyl K8) protein expressing HeLa cells (0.4 µM TSA treatment for 18 hours).
Lane 2: Control with 2.5 μg of pre-immune rabbit IgG.
Lane 3: Immunoprecipitation of Histone H4 (acetyl K9) protein by 2.5 μg of ab227855.
12% SDS-PAGE gel.
The immunoprecipitated Histone H4 (acetyl K9) protein was detected by WB using ab227855 at a 1/5000.
Paraffin-embedded mouse duodenum tissue stained for Histone H4 (acetyl K8) using ab227855 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded mouse kidney tissue stained for Histone H4 (acetyl K8) using ab227855 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded rat hind brain tissue stained for Histone H4 (acetyl K8) using ab227855 at 1/500 dilution in immunohistochemical analysis.
Dot Blot analysis of ab227855 with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with ab227855 at 1/5000 dilution.
A: Peptide samples of H4K8 un
B: Peptide samples of H4K8ac
C: Peptide samples of H4K16un
D: Peptide samples of H4K16ac
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227855 has not yet been referenced specifically in any publications.