Overview

  • Product name

    Anti-Histone H4 (acetyl K8) antibody
    See all Histone H4 primary antibodies
  • Description

    Rabbit polyclonal to Histone H4 (acetyl K8)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ChIP, Dot blot, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Xenopus laevis
  • Immunogen

    Synthetic peptide within Human Histone H4 (N terminal) (acetyl K8). The exact sequence is proprietary. (Carrier-protein conjugated).
    Database link: P62805

  • Positive control

    • ICC/IF: HeLa cells. IHC-P: Rat hind brain tissue. Mouse kidney and duodenum tissue. WB: HeLa treated with DMSO or Trichostatin A, whole cell lysates. IP: HeLa, treated with TSA, whole cell lysate. ChIP: HeLa chromatin extract treated with Trichostatin A.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
IP 1/100 - 1/5000.
IHC-P 1/100 - 1/1000.
ChIP Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-Histone H4 (acetyl K8) antibody (ab227855) at 1/5000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract treated with 0.012% DMSO for 18 hours
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract treated with 0.4 µM Trichostatin A for 18 hours

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 11 kDa



    15% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H4 (acetyl K8) (green) using ab227855 at 1/500 dilution in ICC/IF.

    Red: alpha Tubulin stained with an alpha Tubulin antibody at 1/500 dilution.
    Blue: Hoechst 33342 staining.

  • Cross-linked ChIP was performed with HeLa (human epithelial cell line from cervix adenocarcinoma) chromatin extract treated with Trichostatin A (0.4 μM for 18 hours) and 5 μg of either control rabbit IgG or ab227855. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or TEXT12.

  • Histone H4 (acetyl K8) was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 2.5 µg ab227855. Western blot was performed from the immunoprecipitate using ab227855. Anti-rabbit IgG (HRP) was used as a secondary reagent.

    Lane 1: 30 μg whole cell lysate/extract of Histone H4 (acetyl K8) protein expressing HeLa cells (0.4 µM TSA treatment for 18 hours).

    Lane 2: Control with 2.5 μg of pre-immune rabbit IgG.

    Lane 3: Immunoprecipitation of Histone H4 (acetyl K9) protein by 2.5 μg of ab227855.

    12% SDS-PAGE gel.

    The immunoprecipitated Histone H4 (acetyl K9) protein was detected by WB using ab227855 at a 1/5000.

  • Paraffin-embedded mouse duodenum tissue stained for Histone H4 (acetyl K8) using ab227855 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse kidney tissue stained for Histone H4 (acetyl K8) using ab227855 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat hind brain tissue stained for Histone H4 (acetyl K8) using ab227855 at 1/500 dilution in immunohistochemical analysis.

  • Dot Blot analysis of ab227855 with peptide samples.
    Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with ab227855 at 1/5000 dilution.
    A: Peptide samples of H4K8 un
    B: Peptide samples of H4K8ac
    C: Peptide samples of H4K16un
    D: Peptide samples of H4K16ac

References

ab227855 has not yet been referenced specifically in any publications.

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