Histone H4 (acetyl K8) Quantification Kit (Fluorometric) (ab115119)
- Datasheet
- References
- Protocols
Overview
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Product name
Histone H4 (acetyl K8) Quantification Kit (Fluorometric)
See all Histone H4 kits -
Detection method
Fluorescent -
Sample type
Tissue Extracts, Cell Lysate -
Sensitivity
0.4 ng/well -
Range
20 ng/well - 5000 ng/well -
Assay time
2h 30m -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Mammals
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Product overview
Acetylation of histones such histone H4 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H4 actylation. Histone H4 (acetyl K8) reflects the hyperacetylated stated in histone H4 and strongly correlates with active state of genes. H4 (acetyl K8) is related to DNA repair and increased H4 (acetyl K8) has been observed in cancer and inflammatory diseases.
Abcam's Histone H4 (acetyl K8) Quantification Kit (Fluorometric) (ab115119) allows the experimenter to measure global acetylation of histone H4K8 quickly and consistently. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment and it is suitable for specifically measuring global histone H4K8 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components Identifier 48 tests 96 tests 10X Wash Buffer 1 x 10ml 1 x 20ml 8-Well Sample Strips (with Frame) 6 units 9 units 8-Well Standard Control Strips Green Ringed 2 units 3 units Antibody Buffer 1 x 6ml 1 x 12ml Detecting Antibody, 1 mg/mL 1 x 5µl 1 x 10µl Fluoro Developer 1 x 12µl 1 x 24µl Fluoro Dilution 1 x 4ml 1 x 8ml Fluoro Enhancer 1 x 12µl 1 x 24µl Signal Enhancer 1 x 120µl 1 x 240µl Signal Report Solution 1 x 5µl 1 x 10µl Standard Control, 100 µg/mL 1 x 10µl 1 x 20µl -
Research areas
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Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
Sequence similarities
Belongs to the histone H4 family. -
Post-translational
modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Alternative names
- dJ160A22.1
- dJ160A22.2
- dJ221C16.1
see all -
Database links
- Entrez Gene: 121504 Human
- Entrez Gene: 554313 Human
- Entrez Gene: 8294 Human
- Entrez Gene: 8359 Human
- Entrez Gene: 8360 Human
- Entrez Gene: 8361 Human
- Entrez Gene: 8368 Human
- Entrez Gene: 8370 Human
see all
Associated products
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Related Products
- Histone H4 (acetyl K5) Quantification Kit (Colorimetric) (ab115116)
- Histone H4 (acetyl K5) Quantification Kit (Fluorometric) (ab115117)
- Histone H4 (acetyl K8) Quantification Kit (Colorimetric) (ab115118)
- Histone H4 (acetyl K16) Quantification Kit (Colorimetric) (ab115120)
- Histone H4 (acetyl K16) Quantification Kit (Fluorometric) (ab115121)
- Histone H4 (acetyl K12) Quantification Kit (Colorimetric) (ab115122)
- Histone H4 (acetyl K12) Quantification Kit (Fluorometric) (ab115123)
Protocols
References
ab115119 has not yet been referenced specifically in any publications.
