Overview

  • Product name

    Histone H4 Acetylation Assay Kit
    See all Histone H4 acetylation kits
  • Sample type

    Tissue, Adherent cells, Suspension cells
  • Assay type

    Cell-based (quantitative)
  • Assay time

    5h 00m
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Mammals
  • Product overview

    Acetylation of histones such histone H4 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H4 actylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. Reversible acetylation of nucleosomal histone H4 is believed to correlate with potential transcriptionally activity of eukaryotic chromatin domains.

    Histone H4 Acetylation Assay Kit (ab115103) allows the user to measure global histone H4 acetylation at tremendously fast speeds and consistency, superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The kit is suitable for specifically measuring global histone H4 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.

  • Platform

    Microplate reader

Properties

Protocols

References

ab115103 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us. We have not tested these kits in fish and have no testing data on this use. The kit ab115103 may be suitable for use in fish based on principle. However, the kit ab115124 may not be suitable for fish.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting Abcam.


Regarding the use of a 60 or 100mm plate - this is used for disecting tissue samples if you are using them, however, once you proceed with the histone extraction, the assay is then performed in the strip wells provided with the kit. The strips can be put in the plate frame to analyze on the microplate reader.


I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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