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Anti-Histone H4 antibody (ab134212)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (3)

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Western blot - Anti-Histone H4 antibody (ab134212)
  • Western blot - Anti-Histone H4 antibody (ab134212)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 antibody (ab134212)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody (ab134212)

Key features and details

  • Chicken polyclonal to Histone H4
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human, Recombinant fragment
  • Isotype: IgY

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Overview

  • Product name

    Anti-Histone H4 antibody
    See all Histone H4 primary antibodies
  • Description

    Chicken polyclonal to Histone H4
  • Host species

    Chicken
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Recombinant fragment
    Predicted to work with: Chicken, Cow, Pig, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human Histone H4 aa 50 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: P62805
    (Peptide available as ab13843)

  • Positive control

    • WB: Recombinant Histone H4 protein and NIH3T3, MEF1, PC12 and HeLa cell lysates and HeLa Histone preparation. ICC/IF: methanol fixed HeLa cells. IHC-P: Human breast fibroadenoma tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 3% BSA

    This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgY

Associated products

  • Immunizing Peptide (Blocking)

    • Human Histone H4 peptide (ab13843)
  • Isotype control

    • Chicken IgY - Isotype Control (ab50579)
  • Recombinant Protein

    • Recombinant Human Histone H4 protein (ab198115)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa).
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa).
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities

    Belongs to the histone H4 family.
  • Post-translational
    modifications

    Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
    Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
    Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
    Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
    Sumoylated, which is associated with transcriptional repression.
  • Cellular localization

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P62805 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 318846 Drosophila melanogaster
    • Entrez Gene: 3771854 Drosophila melanogaster
    • Entrez Gene: 3771893 Drosophila melanogaster
    • Entrez Gene: 3771908 Drosophila melanogaster
    • Entrez Gene: 3771935 Drosophila melanogaster
    • Entrez Gene: 3771938 Drosophila melanogaster
    • Entrez Gene: 3771941 Drosophila melanogaster
    • Entrez Gene: 3771947 Drosophila melanogaster
    • Entrez Gene: 3772096 Drosophila melanogaster
    • Entrez Gene: 3772113 Drosophila melanogaster
    • Entrez Gene: 3772129 Drosophila melanogaster
    • Entrez Gene: 3772172 Drosophila melanogaster
    • Entrez Gene: 3772211 Drosophila melanogaster
    • Entrez Gene: 3772254 Drosophila melanogaster
    • Entrez Gene: 3772314 Drosophila melanogaster
    • Entrez Gene: 3772317 Drosophila melanogaster
    • Entrez Gene: 3772319 Drosophila melanogaster
    • Entrez Gene: 3772325 Drosophila melanogaster
    • Entrez Gene: 3772509 Drosophila melanogaster
    • Entrez Gene: 3772519 Drosophila melanogaster
    • Entrez Gene: 3772666 Drosophila melanogaster
    • Entrez Gene: 3772708 Drosophila melanogaster
    • Entrez Gene: 41773 Drosophila melanogaster
    • Entrez Gene: 121504 Human
    • Entrez Gene: 554313 Human
    • Entrez Gene: 8294 Human
    • Entrez Gene: 8359 Human
    • Entrez Gene: 8360 Human
    • Entrez Gene: 8361 Human
    • Entrez Gene: 8362 Human
    • Entrez Gene: 8363 Human
    • Entrez Gene: 8364 Human
    • Entrez Gene: 8365 Human
    • Entrez Gene: 8366 Human
    • Entrez Gene: 8367 Human
    • Entrez Gene: 8368 Human
    • Entrez Gene: 8370 Human
    • Entrez Gene: 100041230 Mouse
    • Entrez Gene: 319155 Mouse
    • Entrez Gene: 319156 Mouse
    • Entrez Gene: 319157 Mouse
    • Entrez Gene: 319158 Mouse
    • Entrez Gene: 319159 Mouse
    • Entrez Gene: 319160 Mouse
    • Entrez Gene: 319161 Mouse
    • Entrez Gene: 320332 Mouse
    • Entrez Gene: 326619 Mouse
    • Entrez Gene: 326620 Mouse
    • Entrez Gene: 674678 Mouse
    • Entrez Gene: 69386 Mouse
    • Entrez Gene: 97122 Mouse
    • Entrez Gene: 64627 Rat
    • Omim: 142750 Human
    • Omim: 602822 Human
    • Omim: 602823 Human
    • Omim: 602824 Human
    • Omim: 602825 Human
    • Omim: 602826 Human
    • Omim: 602827 Human
    • Omim: 602828 Human
    • Omim: 602829 Human
    • Omim: 602830 Human
    • Omim: 602831 Human
    • Omim: 602833 Human
    • SwissProt: P84040 Drosophila melanogaster
    • SwissProt: P62805 Human
    • SwissProt: P62806 Mouse
    • SwissProt: P62804 Rat
    • Unigene: 21500 Drosophila melanogaster
    • Unigene: 29514 Drosophila melanogaster
    • Unigene: 29527 Drosophila melanogaster
    • Unigene: 30219 Drosophila melanogaster
    • Unigene: 30220 Drosophila melanogaster
    • Unigene: 30221 Drosophila melanogaster
    • Unigene: 30223 Drosophila melanogaster
    • Unigene: 30868 Drosophila melanogaster
    • Unigene: 30869 Drosophila melanogaster
    • Unigene: 30871 Drosophila melanogaster
    • Unigene: 30872 Drosophila melanogaster
    • Unigene: 30873 Drosophila melanogaster
    • Unigene: 30876 Drosophila melanogaster
    • Unigene: 33873 Drosophila melanogaster
    • Unigene: 5747 Drosophila melanogaster
    • Unigene: 143080 Human
    • Unigene: 247816 Human
    • Unigene: 248172 Human
    • Unigene: 248178 Human
    • Unigene: 248179 Human
    • Unigene: 278483 Human
    • Unigene: 46423 Human
    • Unigene: 528055 Human
    • Unigene: 533295 Human
    • Unigene: 55468 Human
    • Unigene: 591790 Human
    • Unigene: 655235 Human
    • Unigene: 662174 Human
    • Unigene: 706635 Human
    • Unigene: 718935 Human
    • Unigene: 144300 Mouse
    • Unigene: 14775 Mouse
    • Unigene: 158272 Mouse
    • Unigene: 227295 Mouse
    • Unigene: 228709 Mouse
    • Unigene: 246720 Mouse
    • Unigene: 255646 Mouse
    • Unigene: 260530 Mouse
    • Unigene: 261642 Mouse
    • Unigene: 261662 Mouse
    • Unigene: 261664 Mouse
    • Unigene: 377875 Mouse
    • Unigene: 442307 Mouse
    see all
  • Alternative names

    • dJ160A22.1 antibody
    • dJ160A22.2 antibody
    • dJ221C16.1 antibody
    • dJ221C16.9 antibody
    • FO108 antibody
    • H4 antibody
    • H4.k antibody
    • H4/a antibody
    • H4/b antibody
    • H4/c antibody
    • H4/d antibody
    • H4/e antibody
    • H4/g antibody
    • H4/h antibody
    • H4/I antibody
    • H4/j antibody
    • H4/k antibody
    • H4/m antibody
    • H4/n antibody
    • H4/p antibody
    • H4_HUMAN antibody
    • H4F2 antibody
    • H4F2iii antibody
    • H4F2iv antibody
    • H4FA antibody
    • H4FB antibody
    • H4FC antibody
    • H4FD antibody
    • H4FE antibody
    • H4FG antibody
    • H4FH antibody
    • H4FI antibody
    • H4FJ antibody
    • H4FK antibody
    • H4FM antibody
    • H4FN antibody
    • H4M antibody
    • HIST1H4A antibody
    • HIST1H4B antibody
    • HIST1H4C antibody
    • HIST1H4D antibody
    • HIST1H4E antibody
    • HIST1H4F antibody
    • HIST1H4H antibody
    • HIST1H4I antibody
    • HIST1H4J antibody
    • HIST1H4K antibody
    • HIST1H4L antibody
    • HIST2H4 antibody
    • HIST2H4A antibody
    • Hist4h4 antibody
    • Histone 1 H4a antibody
    • Histone 1 H4b antibody
    • Histone 1 H4c antibody
    • Histone 1 H4d antibody
    • Histone 1 H4e antibody
    • Histone 1 H4f antibody
    • Histone 1 H4h antibody
    • Histone 1 H4i antibody
    • Histone 1 H4j antibody
    • Histone 1 H4k antibody
    • Histone 1 H4l antibody
    • Histone 2 H4a antibody
    • histone 4 H4 antibody
    • Histone H4 antibody
    • MGC24116 antibody
    see all

Images

  • Western blot - Anti-Histone H4 antibody (ab134212)
    Western blot - Anti-Histone H4 antibody (ab134212)
    All lanes : Anti-Histone H4 antibody (ab134212) at 1 µg/ml

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
    Lane 5 : HeLa Histone Preparation Nuclear Lysate at 2.5 µg
    Lane 6 : Histone H4 Recombinant Protein at 0.1 µg
    Lane 7 : Histone H3.1 Recombinant Protein (negative control) at 0.1 µg

    Secondary
    All lanes : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 11 kDa


    Exposure time: 3 minutes
  • Western blot - Anti-Histone H4 antibody (ab134212)
    Western blot - Anti-Histone H4 antibody (ab134212)
    All lanes : Anti-Histone H4 antibody (ab134212) at 1 µg/ml

    Lane 1 : Histone H4 Recombinant Protein
    Lane 2 : Histone H2A Recombinant Protein

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 14 kDa why is the actual band size different from the predicted?


    Exposure time: 8 minutes
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 antibody (ab134212)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 antibody (ab134212)

    ICC/IF image of ab134212 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab134212 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- chicken IgY (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody (ab134212)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody (ab134212)
    IHC image of Histone H4 staining in human breast fibroadenoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab134212, 5µg/ml, for 15 mins at room temperature. A goat anti-chicken biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (3)

Publishing research using ab134212? Please let us know so that we can cite the reference in this datasheet.

ab134212 has been referenced in 3 publications.

  • Ginno PA  et al. Cell cycle-resolved chromatin proteomics reveals the extent of mitotic preservation of the genomic regulatory landscape. Nat Commun 9:4048 (2018). PubMed: 30279501
  • Ganatra HA  et al. Zinc supplementation leads to immune modulation and improved survival in a juvenile model of murine sepsis. Innate Immun 23:67-76 (2017). PubMed: 27821649
  • Liu Y  et al. MDM2-MOF-H4K16ac axis contributes to tumorigenesis induced by Notch. FEBS J 281:3315-24 (2014). PubMed: 24898892

Customer reviews and Q&As

Show All Reviews Q&A
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Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Histone H4 antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (small intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate based, VECTOR H3300
Specification
small intestine
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Dec 11 2017

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