Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T cells cultured in DMEME+10% FBS)
Gel Running Conditions
Reduced Denaturing (15% precast Bis-Tris gel)
Loading amount
20 µg
Treatment
H3.3 with V5 tag over-expressed in pTRIEX 3 plasmid. Harvested 48h post-transfection
Specification
HEK293T cells cultured in DMEME+10% FBS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% milk in TBST buffer
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Immun-Star Goat Anti-Rabbit (GAR)-HRP # 170-5046
Host species: Human
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Human
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL reagent
Exposure
45 second(s)
Bands
Specific: 14 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ranveer Jayani
Verified customer
Submitted Dec 19 2017