Western blot abreview for Anti-Histone H4 antibody [EPR16599] - ChIP Grade

Good
Abreviews
Application
Western blot
Sample
Human Recombinant protein (Recombinant octamers & purified K-562 cell histone)
Gel Running Conditions
Reduced Denaturing (18% gel)
Loading amount
0.5 µg
Specification
Recombinant octamers & purified K-562 cell histone
Blocking step
Odyssey blocking solution 1/1 (vol/vol) PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Donkey-anti-rabbit-IRDYE 800
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
Odyssey Imaging system channel 800
Negative control
All other histones.
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 13 kDa
Positive control
Drosophila and Human Histone H3,recombinant and native

Additional data

Additional Notes
Lane 1. K562 octamer 1 microgram, Lane 2. K562 octamer 500 nanograms, Lane 3. Recombinant Human octamer 500 nanograms, Lane 4. Recombinant Drosophila octamer 500 nanograms.

Dr. Ragnhild Eskeland

Verified customer

Submitted May 11 2015

Sign up