Recombinant
RabMAb

Recombinant Anti-Histone H4 antibody [EPR16599] (HRP) (ab208870)

Overview

  • Product name
    Anti-Histone H4 antibody [EPR16599] (HRP)
    See all Histone H4 primary antibodies
  • Description
    Rabbit monoclonal [EPR16599] to Histone H4 (HRP)
  • Host species
    Rabbit
  • Conjugation
    HRP
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Drosophila melanogaster
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Histone H4 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: P62805

  • Positive control
    • WB: Histone H4 recombinant protein. IHC-P: normal human colon tissue sections
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer
    pH: 7.4
    Preservative: 0.1% Proclin
    Constituents: PBS, 1% BSA, 30% Glycerol
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR16599
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab208870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa).

Target

Images

  • IHC image of Histone H4 staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab208870, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-Histone H4 antibody [EPR16599] (HRP) (ab208870) at 1/5000 dilution

    Lane 1 : Histone H1 Recombinant Protein
    Lane 2 : Histone H2A Recombinant Protein
    Lane 3 : Histone H2B Recombinant Protein
    Lane 4 : Histone H3 Recombinant Protein
    Lane 5 : Histone H4 Recombinant Protein

    Lysates/proteins at 0.1 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 14 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab208870 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab208870 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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