Recombinant

Recombinant Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Human monoclonal [IGX4696H] to Histone H4 - ChIP Grade
  • Suitable for: WB, ChIP
  • Reacts with: Mouse, Human, Recombinant fragment

Overview

  • Product name

    Anti-Histone H4 antibody [IGX4696H] - ChIP Grade
    See all Histone H4 primary antibodies

  • Description

    Human monoclonal [IGX4696H] to Histone H4 - ChIP Grade

  • Host species

    Human

  • Tested applications

    Suitable for: WB, ChIPmore details

  • Species reactivity

    Reacts with: Mouse, Human, Recombinant fragment

  • Immunogen

    Synthetic peptide.
    Database link: P62805

  • Positive control

    • WB: CTH, HeLa whole cell lysate, HeLa nuclear lysate, NIH3T3 whole cell lysate, NIH3T3 nuclear lysate and Histone H4B Recombinant Protein. ChIP: HeLa and NIH3T3.

  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant antibody. 
    Human monoclonal antibody.

     

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Monoclonal

  • Clone number

    IGX4696H

  • Isotype

    IgG1

Applications

Our Abpromise guarantee covers the use of ab213291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa).
ChIP Use 2 µg for 25 µg of chromatin.

Target

Images

  • Western blot - Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)
    Western blot - Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)
    All lanes : Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291) at 0.2 µg/ml

    Lane 1 : CTH (Calf Thymus Histone) at 0.5 µg
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg
    Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg
    Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Nuclear Lysate at 10 µg
    Lane 6 : Histone H2A Recombinant Protein at 0.1 µg
    Lane 7 : Histone H4 Recombinant Protein at 0.1 µg

    Secondary
    All lanes : HRP conjugated Goat Anti-Human IgG (H+L) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 14 kDa
    why is the actual band size different from the predicted?


    Exposure time: 4 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab213291 overnight at 4°C. Antibody binding was detected using an anti-human antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • ChIP - Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)
    ChIP - Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)

    Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab213291 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

  • ChIP - Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)
    ChIP - Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)

    Chromatin was prepared from NIH3T3 cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab213291 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach). Primers and probes are located in the first kb of the transcribed region.

References (0)

Publishing research using ab213291? Please let us know so that we can cite the reference in this datasheet.

ab213291 has not yet been referenced specifically in any publications.

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