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    histone-h4-antibody-igx4696h-chip-grade-ab213291.pdf

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Recombinant

Recombinant Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)

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Western blot - Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)
  • ChIP - Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)
  • ChIP - Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)
  • Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Human monoclonal [IGX4696H] to Histone H4 - ChIP Grade
  • Suitable for: WB, ChIP
  • Reacts with: Mouse, Cow, Human, Recombinant fragment

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Overview

  • Product name

    Anti-Histone H4 antibody [IGX4696H] - ChIP Grade
    See all Histone H4 primary antibodies
  • Description

    Human monoclonal [IGX4696H] to Histone H4 - ChIP Grade
  • Host species

    Human
  • Tested applications

    Suitable for: WB, ChIPmore details
  • Species reactivity

    Reacts with: Mouse, Cow, Human, Recombinant fragment
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: CTH, HeLa whole cell lysate, HeLa nuclear lysate, NIH3T3 whole cell lysate, NIH3T3 nuclear lysate and Histone H4B Recombinant Protein. ChIP: HeLa and NIH3T3.
  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant antibody. 
    Human monoclonal antibody.

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    IGX4696H
  • Isotype

    IgG1

Associated products

  • Compatible Secondaries

    • Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Alexa Fluor® 488) (ab200622)
    • Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Alexa Fluor® 647) (ab200623)
    • Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Biotin) (ab201485)
    • Rabbit Anti-Human IgG H&L (HRP) (ab6759)
  • Isotype control

    • Human IgG1, kappa - Isotype Control (ab206198)
  • Recombinant Protein

    • Recombinant Human Histone H4 protein (ab198115)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab213291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 0.2 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa).
ChIP
Use 2 µg for 25 µg of chromatin.
Notes
WB
Use a concentration of 0.2 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa).
ChIP
Use 2 µg for 25 µg of chromatin.

Target

  • Function

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities

    Belongs to the histone H4 family.
  • Post-translational
    modifications

    Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
    Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
    Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
    Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
    Sumoylated, which is associated with transcriptional repression.
  • Cellular localization

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P62805 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 121504 Human
    • Entrez Gene: 554313 Human
    • Entrez Gene: 8294 Human
    • Entrez Gene: 8359 Human
    • Entrez Gene: 8360 Human
    • Entrez Gene: 8361 Human
    • Entrez Gene: 8362 Human
    • Entrez Gene: 8363 Human
    • Entrez Gene: 8364 Human
    • Entrez Gene: 8365 Human
    • Entrez Gene: 8366 Human
    • Entrez Gene: 8367 Human
    • Entrez Gene: 8368 Human
    • Entrez Gene: 8370 Human
    • Entrez Gene: 100041230 Mouse
    • Entrez Gene: 319155 Mouse
    • Entrez Gene: 319156 Mouse
    • Entrez Gene: 319157 Mouse
    • Entrez Gene: 319158 Mouse
    • Entrez Gene: 319159 Mouse
    • Entrez Gene: 319160 Mouse
    • Entrez Gene: 319161 Mouse
    • Entrez Gene: 320332 Mouse
    • Entrez Gene: 326619 Mouse
    • Entrez Gene: 326620 Mouse
    • Entrez Gene: 674678 Mouse
    • Entrez Gene: 69386 Mouse
    • Entrez Gene: 97122 Mouse
    • Omim: 142750 Human
    • Omim: 602822 Human
    • Omim: 602823 Human
    • Omim: 602824 Human
    • Omim: 602825 Human
    • Omim: 602826 Human
    • Omim: 602827 Human
    • Omim: 602828 Human
    • Omim: 602829 Human
    • Omim: 602830 Human
    • Omim: 602831 Human
    • Omim: 602833 Human
    • SwissProt: P62805 Human
    • SwissProt: P62806 Mouse
    • Unigene: 143080 Human
    • Unigene: 247816 Human
    • Unigene: 248172 Human
    • Unigene: 248178 Human
    • Unigene: 248179 Human
    • Unigene: 278483 Human
    • Unigene: 46423 Human
    • Unigene: 528055 Human
    • Unigene: 533295 Human
    • Unigene: 55468 Human
    • Unigene: 591790 Human
    • Unigene: 655235 Human
    • Unigene: 662174 Human
    • Unigene: 706635 Human
    • Unigene: 718935 Human
    • Unigene: 144300 Mouse
    • Unigene: 14775 Mouse
    • Unigene: 158272 Mouse
    • Unigene: 227295 Mouse
    • Unigene: 228709 Mouse
    • Unigene: 246720 Mouse
    • Unigene: 255646 Mouse
    • Unigene: 260530 Mouse
    • Unigene: 261642 Mouse
    • Unigene: 261662 Mouse
    • Unigene: 261664 Mouse
    • Unigene: 377875 Mouse
    • Unigene: 442307 Mouse
    see all
  • Alternative names

    • dJ160A22.1 antibody
    • dJ160A22.2 antibody
    • dJ221C16.1 antibody
    • dJ221C16.9 antibody
    • FO108 antibody
    • H4 antibody
    • H4.k antibody
    • H4/a antibody
    • H4/b antibody
    • H4/c antibody
    • H4/d antibody
    • H4/e antibody
    • H4/g antibody
    • H4/h antibody
    • H4/I antibody
    • H4/j antibody
    • H4/k antibody
    • H4/m antibody
    • H4/n antibody
    • H4/p antibody
    • H4_HUMAN antibody
    • H4F2 antibody
    • H4F2iii antibody
    • H4F2iv antibody
    • H4FA antibody
    • H4FB antibody
    • H4FC antibody
    • H4FD antibody
    • H4FE antibody
    • H4FG antibody
    • H4FH antibody
    • H4FI antibody
    • H4FJ antibody
    • H4FK antibody
    • H4FM antibody
    • H4FN antibody
    • H4M antibody
    • HIST1H4A antibody
    • HIST1H4B antibody
    • HIST1H4C antibody
    • HIST1H4D antibody
    • HIST1H4E antibody
    • HIST1H4F antibody
    • HIST1H4H antibody
    • HIST1H4I antibody
    • HIST1H4J antibody
    • HIST1H4K antibody
    • HIST1H4L antibody
    • HIST2H4 antibody
    • HIST2H4A antibody
    • Hist4h4 antibody
    • Histone 1 H4a antibody
    • Histone 1 H4b antibody
    • Histone 1 H4c antibody
    • Histone 1 H4d antibody
    • Histone 1 H4e antibody
    • Histone 1 H4f antibody
    • Histone 1 H4h antibody
    • Histone 1 H4i antibody
    • Histone 1 H4j antibody
    • Histone 1 H4k antibody
    • Histone 1 H4l antibody
    • Histone 2 H4a antibody
    • histone 4 H4 antibody
    • Histone H4 antibody
    • MGC24116 antibody
    see all

Images

  • Western blot - Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)
    Western blot - Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)
    All lanes : Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291) at 0.2 µg/ml

    Lane 1 : CTH (Calf Thymus Histone) at 0.5 µg
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg
    Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg
    Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Nuclear Lysate at 10 µg
    Lane 6 : Histone H2A Recombinant Protein at 0.1 µg
    Lane 7 : Histone H4 Recombinant Protein at 0.1 µg

    Secondary
    All lanes : HRP conjugated Goat Anti-Human IgG (H+L) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 14 kDa why is the actual band size different from the predicted?


    Exposure time: 4 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab213291 overnight at 4°C. Antibody binding was detected using an anti-human antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • ChIP - Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)
    ChIP - Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)

    Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab213291 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

  • ChIP - Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)
    ChIP - Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)

    Chromatin was prepared from NIH3T3 cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab213291 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach). Primers and probes are located in the first kb of the transcribed region.

  • Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)
    Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)

Protocols

  • ChIP protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab213291? Please let us know so that we can cite the reference in this datasheet.

ab213291 has not yet been referenced specifically in any publications.

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