Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade – BSA and Azide free (ab174628)

Overview

  • Product name

    Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade – BSA and Azide free
    See all Histone H4 primary antibodies
  • Description

    Mouse monoclonal [mAbcam 17036] to Histone H4 - ChIP Grade – BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, ChIP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic peptide corresponding to Human Histone H4 aa 50 to the C-terminus. Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Histone H4.

    Read Abcam's proprietary immunogen policy (Peptide available as ab13843.)
    Database link: P62805

  • Positive control

    • This antibody gave a positive signal in the following: Calf thymus histone preparation; HeLa histone preparation; Histone H4 recombinant protein; NIH3T3 Whole Cell Lysate; MEF1 Whole Cell Lysate; PC12 Whole Cell Lysate.
  • General notes

    This antibody clone is manufactured by Abcam.

    This product is the PBS-only buffer version of ab17036.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituent: PBS
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    mAbcam 17036
  • Myeloma

    Sp2/0-Ag14
  • Isotype

    IgG2a
  • Light chain type

    kappa

Applications

Our Abpromise guarantee covers the use of ab174628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml.
ChIP Use 5 µg for 25 µg of chromatin.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 11 kDa.

Target

Images

  • This ChIP data was generated using the same antibody clone in a different buffer formulation (ab17036). 

    Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab17036 (blue), and 20µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

References

This product has been referenced in:

  • Liu Y  et al. Structural insights into trans-histone regulation of H3K4 methylation by unique histone H4 binding of MLL3/4. Nat Commun 10:36 (2019). Read more (PubMed: 30604749) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
African green monkey Cell lysate - whole cell (Cos7)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
10 µg
Specification
Cos7
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Aug 28 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up