Key features and details
- Mouse monoclonal [mAbcam 17036] to Histone H4 - ChIP Grade – BSA and Azide free
- Suitable for: IHC-P, ChIP, WB
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2a
Product nameAnti-Histone H4 antibody [mAbcam 17036] - ChIP Grade – BSA and Azide free
See all Histone H4 primary antibodies
DescriptionMouse monoclonal [mAbcam 17036] to Histone H4 - ChIP Grade – BSA and Azide free
Tested applicationsSuitable for: IHC-P, ChIP, WBmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human
- This antibody gave a positive signal in the following: Calf thymus histone preparation; HeLa histone preparation; Histone H4 recombinant protein; NIH3T3 Whole Cell Lysate; MEF1 Whole Cell Lysate; PC12 Whole Cell Lysate.
This antibody clone is manufactured by Abcam.
This product is the PBS-only buffer version of ab17036.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Concentration information loading...
Clone numbermAbcam 17036
Light chain typekappa
Our Abpromise guarantee covers the use of ab174628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml.|
|ChIP||Use 5 µg for 25 µg of chromatin.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 11 kDa.|
FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similaritiesBelongs to the histone H4 family.
modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
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This ChIP data was generated using the same antibody clone in a different buffer formulation (ab17036).
Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab17036 (blue), and 20µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
ab174628 has been referenced in 2 publications.
- Szewczyk MM et al. Pharmacological inhibition of PRMT7 links arginine monomethylation to the cellular stress response. Nat Commun 11:2396 (2020). PubMed: 32409666
- Liu Y et al. Structural insights into trans-histone regulation of H3K4 methylation by unique histone H4 binding of MLL3/4. Nat Commun 10:36 (2019). PubMed: 30604749